FastQCFastQC Report
Fri 20 Dec 2024
2G1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2G1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17345549
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC6680273.8512877280505795Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3962902.2846783344822352No Hit
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC3750432.1621858149315427Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC2492701.4370833693416103Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1806161.0412815414490484No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT1404400.8096601612321409No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC1204860.6946220036044981No Hit
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT1033940.5960837561267158Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCATATTCTGTTCTTTCTAACTCTGAAAGCAGATCGGAAGAGCACACGTC989740.5706017145954849No Hit
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA862460.4972226592539677No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC819010.4721730053052803No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGGGGG701990.4047090120929583TruSeq Adapter, Index 7 (97% over 45bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA678550.3911954588465318Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA678470.3911493375043938No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG617160.35580309392340365Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA612560.35315111675047006Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC532930.3072430858198838Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT525620.3030287481820264Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT500220.28838522205321954No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAGATCGGAAGAGCACA461680.26616626547825034No Hit
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA450460.25969774724339945No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG432300.24922820257807926Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAACGTAAAATCTGAACAAATCTTGTTCAGATCGGAAGAGCACACGTCTG418300.24115696770393374Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAGATCGGAAGAGCACAC405880.2339966293370132No Hit
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAA404120.23298195980997774No Hit
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA399730.2304510511601564No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGA399280.23019161861063034No Hit
GCACTGGTGGTTCAATGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT375020.21620532160728959No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC349880.2017116898404311No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAG349670.20159062131731892No Hit
CACCCCTCCCCGCACATTGACCTCTTAAGATCGGAAGAGCACACGTCTGA323080.1862610402242097Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTTTATGCTGTAGGTAAAAGCCCAAAAAAAAGATCGGAAGAGCACACG320990.1850561201608551No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG314810.18149324648069656No Hit
TGAAGACGAAGCTCAACCTGAGGAAAATCAGATCGGAAGAGCACACGTCT306990.17698488528670958Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG304620.17561854052587209Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAG293800.16938062900171105No Hit
GAGGTCCGGACTTGGGGAGGGTTATCAGATCGGAAGAGCACACGTCTGAA289250.16675747766761376Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA279330.16103843124250491Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGCGATCTATTGAGACTCAACCTTTTGACGGGGAGATTTGAAGATCGG267620.1542874197870589No Hit
TACGATTTGGCGATACTACATCTGAAGTAGCAGATCGGAAGAGCACACGT257460.14843000933553616No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTGGG254730.1468561185350778TruSeq Adapter, Index 7 (97% over 46bp)
TCTCATGGAACTTCGAAGTACATATTCAGCAGATCGGAAGAGCACACGTC228150.1315323026097358No Hit
TTACATCTACCTTTGAAAAATTCGAAAAGTAGATCGGAAGAGCACACGTC227200.13098461167184733No Hit
TCAGCAAATATTTCCGACTGCTTGATGAGCAGATCGGAAGAGCACACGTC226400.1305233982504676No Hit
GTGCTAACGATTTGAAGTCTCTGGATTTTAGATCGGAAGAGCACACGTCT226330.13048304207609687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAAC217540.1254154596086869Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC215030.12396840249910797No Hit
ATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAAGAGCACA211600.12199094995494232No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC211230.12177763874755419No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCAAGATCGGAAGAGCACACGTCTG207030.11935626828531054Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTGTCTGAATTCAACTCTGAACCGTTGTCAGATCGGAAGAGCACACGT201040.1159029327927297No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACCCTTAAGATCGGAAGAGCACAC200510.11559737890106563No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTAGATCGGAAGAGCACACG198100.11420797346915915No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG195370.11263408266870077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGGGGG193640.11163670864496708TruSeq Adapter, Index 7 (97% over 45bp)
GTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGG192700.11109478287484587No Hit
ACCCCTCCCCGCACATTGACCTCTTAAGATCGGAAGAGCACACGTCTGAA191070.11015506052878465Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAAAATCTTTCTTCTGAGGCGGAAGATCGGAAGAGCACACGTCTGAACT190510.10983221113381883Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC186050.10726094630962675No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGAGATCGGAAGAGCACACGTCTG184640.10644805765444496Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTATGGAATTTAAGGGACATACGACGAGTAGATCGGAAGAGCACACGTCT182580.1052604330943921Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGTGGTTCAATGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC181730.10477039383417613No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT181510.1046435601432967No Hit
TAAAACTTGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA176320.10165143807209562TruSeq Adapter, Index 7 (100% over 37bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG174800.10077513257147411No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)