FastQCFastQC Report
Fri 20 Dec 2024
2F1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2F1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19547489
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT5756472.944864171556766Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2747851.4057304239946113No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG1713660.8766650284340869Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCAGATCGTCGGACTG1381670.7068273577235419No Hit
GAGAATTCTACCACTGAACCACCAGTGATCGTCGGACTGTAGAACTCTGA1334930.6829163582084635Illumina DpnII expression Sequencing Primer (100% over 24bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGATC1141980.5842080279467097No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT800330.409428546039852No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC714460.365499630157101No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATGATCGTCGGACT615230.31473607684342475No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG528540.270387669741111Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAGAATTCTACCACTGAACCACAGTGATCGTCGGACTGTAGAACTCTGAA478030.24454803376535986Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG400300.20478333559875642Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC390450.19974432521742305No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC372060.19033646725674075No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA304420.15573355738939154No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT286360.14649451906585037Illumina RNA PCR Primer (100% over 50bp)
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC271710.1389999503261007No Hit
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT270280.13826839856515585No Hit
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA245290.12548414786165119Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACGGTCACCGACGAATCGGATCGTCGGACTGTAGAACTCTGAACGTGTA241750.12367317357231918Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGAATTCTACCACTGAACCACCAGGATCGTCGGACTGTAGAACTCTGAA233180.11928897875323015Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCCGATCGTCGGA228830.11706362899091541No Hit
GCTCGTCCCAATGTAGAGAGCGAGGTCTCGTTCGTTCACGGGATCGTCGG227180.11621953080520982No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG217780.11141072901997796No Hit
AAGCCTACGACACGGGGTATTCCCAGGATCGTCGGACTGTAGAACTCTGA215320.11015225536128964Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT208430.10662750596764628No Hit
GAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCAGATCGTCGGACTG200820.1027344228202405No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)