FastQCFastQC Report
Fri 20 Dec 2024
2F1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2F1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19547489
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG5699242.915586753879232Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2758101.410974064239146No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA1692650.8659168448694357Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGC1370330.7010261011017834No Hit
ACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGAA1327060.6788902656499768Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGAT1136150.5812255476905499No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC797500.40798078975770236No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT719270.36796030426209725No Hit
ATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAG610620.31237771767002914No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA517920.26495474687311504Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGAAC475910.24346349549039265Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA396160.20266541651462242Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT389280.1991457828675591No Hit
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA369640.18909845658437255No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC303180.15509920481346734No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC271590.1389385613671403No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCAGATCGGAAGAGCACACGT268480.13734756418074975No Hit
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT239910.12273187620159295Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGATTCGTCGGTGACCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT239110.12232261647519024Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGAAC232210.11879275133496686Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAG227660.11646508664105144No Hit
CCGTGAACGAACGAGACCTCGCTCTCTACATTGGGACGAGCAGATCGGAA227570.11641904492183112No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC215130.11005505617626898No Hit
CTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCACACGTCTGAA211940.10842313301723817Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC210070.10746648840677185No Hit
TGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTCAGATCGGAAGAGC202800.10374734064308719No Hit
GACGGGCCGGTCGTCGGCGACGTCGACCTTGAACAAATCGAAGTGCTCCA198320.10145548617523203No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)