FastQCFastQC Report
Fri 20 Dec 2024
2E2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2E2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21855244
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC13561836.205297913855365No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA5785042.646980285372243No Hit
AACGCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAG5083502.3259863856930627No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC5003392.2893315672888392No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA3581031.638522086507019No Hit
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC1813000.8295491919467932No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1753400.8022788489572571No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1601200.7326388119940459No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA1497320.6851078853203377No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1441970.6597821557151227Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG1410000.645154087504125No Hit
GCAGTCGAATGCTCTACCACTGAGCTATACCCCCGATCGTCGGACTGTAG1072510.49073348254542476No Hit
AGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAACTC967010.44246131500522257Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA949090.4342619098647446No Hit
GGCAGCTATGCTCACCACTATACCACCAACGCGATCGTCGGACTGTAGAA916920.41954233043566114No Hit
GGCAGCTATGCTCACCACTATACCACCGACGCGATCGTCGGACTGTAGAA899690.4116586389975788No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT827290.3785315780505585Illumina RNA PCR Primer (100% over 50bp)
ACAACGCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGT811550.37132964518721456No Hit
CAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAACT802880.36736263388319984No Hit
GACGCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAG754230.3451025300838554No Hit
GGTAAAGCTTACATAAGATTTCTTCATCCATGATCGTCGGACTGTAGAAC746670.34164340604021626No Hit
ACTCTGCGGCGTACAATACCTCGTTCTCTTGATCGTCGGACTGTAGAACT707000.32349215593291936No Hit
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA692040.31664711682010965Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA686710.31420834285812593No Hit
GGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA666350.30489250085700254No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT651480.2980886417923314No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT637800.29182927447527013No Hit
AGTCGAATGCTCTACCACTGAGCTATACCCCCGATCGTCGGACTGTAGAA633380.2898068765555763No Hit
AGTCTGATGCTCTACCGACTGAGCTAACCGGGCGATCGTCGGACTGTAGA621470.28435738351857337No Hit
AGCCCGACGACTTACCCTTCGTCCTCTCTACGATCGTCGGACTGTAGAAC550960.25209510358246284No Hit
AACGCAGGGTCCTAACCACTAGACGATCGCGGCGATCGTCGGACTGTAGA531650.24325969547628937No Hit
ATCCAAGTACTAACCCCGCCCGACAGAGCTTAACTTCGATCGTCGGACTG496330.22709881436235624No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAAC496120.2270027275833663No Hit
CATCCAAGTACTAACCCCGCCCGACAGAGCTTAACTTCGATCGTCGGACT484550.22170880361710904No Hit
TACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTAGAACT475850.21772806563038144No Hit
AAAGCTTACATAAGATTTCTTCATCCATGATCGTCGGACTGTAGAACTCT473190.21651096642984172Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGT462150.21145954719151158Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTCGGCGGGTGATCCAGGCTGATCCGAGGTCCGGATCGTCGGACTGTA451060.20638525014865997No Hit
AGGCGGGGATACTCACCACTATACTACCGAGGAGATCGTCGGACTGTAGA447220.2046282347614147No Hit
ACAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC439510.20110047730421127No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG428350.1959941513350297No Hit
GGCGGGGATACTCACCACTATACTACCGAGGAGATCGTCGGACTGTAGAA418680.1915695839405865No Hit
GCCGGATCCTAACCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT406940.18619787543895644No Hit
AGCAGCTATGCTCACCACTATACCACCAACGCGATCGTCGGACTGTAGAA375490.17180773639498145No Hit
GGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA366920.16788648070000955Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCAGCTATGCTCACCACTATACCACCGACGCGATCGTCGGACTGTAGAA365770.16736029119601686No Hit
ACAGGCGGGGATACTCACCACTATACTACCGAGGAGATCGTCGGACTGTA360980.16516859752286453No Hit
TGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA357950.1637822025688663No Hit
GCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTAG351510.16083554134650704Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCAGGAATCCTAACCGCTAGACCATATGGGAGATCGTCGGACTGTAGA347050.15879484118319612No Hit
GGGATACTCACCACTATACTACCGAGGAGATCGTCGGACTGTAGAACTCT344310.15754113749542217Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG312890.14316472513416Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAGTCGAATGCTCTACCACTGAGCTATACCCCCGATCGTCGGACTGTAG312790.14311896952511718No Hit
AGCGCCGGATCCTAACCACTAGACCATATGGGAGATCGTCGGACTGTAGA311080.14233654861048453No Hit
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA311040.14231824636686738No Hit
AGCTTACATAAGATTTCTTCATCCATGATCGTCGGACTGTAGAACTCTGA304290.1392297427564753Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCTGCACTCCACACCAGTGAAGCTCATCCGCGCGATCGTCGGACTGTAG298740.13669030645459734No Hit
GGCAGCTGTGCTCACCACTATACCACCGACGCGATCGTCGGACTGTAGAA283090.12952955363939198No Hit
ACTGCTCTACCGCTGAGCTAATCCGGAGATCGTCGGACTGTAGAACTCTG274250.12548475780000443Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGGCAGCTATGCTCACCACTATACCACCGACGCGATCGTCGGACTGTAGA264780.12115170162364695No Hit
GTCTGATGCTCTACCGACTGAGCTAACCGGGCGATCGTCGGACTGTAGAA262420.12007186925023577No Hit
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC259270.11863056756538613Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA257750.11793508230793488No Hit
GAAGCCTGCACTCCACACCAGTGAAGCTCATCCGCGCGATCGTCGGACTG256690.11745007285208073No Hit
AGGAATCCTAACCGCTAGACCATATGGGAGATCGTCGGACTGTAGAACTC256660.11743634616936786Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC255430.11687355217814085No Hit
AGAACCGGTGTTTTCCCTGTGGTATGGTCGTAGACGATCGTCGGACTGTA231430.1058922060078579No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT226090.10344885648496992No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG222800.10194349694746031Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGA219670.10051134638441922Illumina DpnII expression Sequencing Primer (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)