FastQCFastQC Report
Fri 20 Dec 2024
2E2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2E2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21855244
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT12992095.944609906894657No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC5570912.5490037997287973No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT4905742.2446512150585005No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCGTTAGATCGGAAGAGCACA4859002.2232650433918746No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG3441701.5747707964276219No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCAGATCGGAAGAGCACACGT1721080.7874906361146093No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1670900.7645304714969093No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1551600.7099440299087943No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1434590.6564053917677606No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA1360960.6227155368295133No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1353950.6195080686356098Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGTATAGCTCAGTGGTAGAGCATTCGACTGCAGATCGGAAGAGCACA1018420.4659842736141495No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCAGATCGGAAGAGCACAC943740.43181398478095234No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTAGATCGGAAGAGCACACGTCT919230.42059928500455085Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCAGATCGGAAGAGCACACG855120.39126536404718243No Hit
GCGTCGGTGGTATAGTGGTGAGCATAGCTGCCAGATCGGAAGAGCACACG843700.3860400734944895No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCGTTGTAGATCGGAAGAGCA774270.3542719541360417No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCGTCAGATCGGAAGAGCACA742720.33983605948302387No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGAGATCGGAAGAGCACACGTC742120.33956152582876675No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACCAGATCGGAAGAGCACACGT709160.3244804770882448No Hit
AAGAGAACGAGGTATTGTACGCCGCAGAGTAGATCGGAAGAGCACACGTC672820.3078528887620747No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG665300.3044120669620527No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC663020.3033688390758758No Hit
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT637340.291618798673673Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC636830.2913854450675545No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC607880.2781391962496506No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTGGGGGGGGGGG606720.27760843118475365TruSeq Adapter, Index 13 (97% over 39bp)
GGGGGTATAGCTCAGTGGTAGAGCATTCGACTAGATCGGAAGAGCACACG603320.2760527404772969No Hit
GCCCGGTTAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC597120.2732158927166405No Hit
GTAGAGAGGACGAAGGGTAAGTCGTCGGGCTAGATCGGAAGAGCACACGT524220.23986005372440589No Hit
GCCGCGATCGTCTAGTGGTTAGGACCCTGCGTTAGATCGGAAGAGCACAC501950.22967027959056416No Hit
GAAGTTAAGCTCTGTCGGGCGGGGTTAGTACTTGGATAGATCGGAAGAGC476290.21792939031016995No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT475740.21767773446043429No Hit
GAAGTTAAGCTCTGTCGGGCGGGGTTAGTACTTGGATGAGATCGGAAGAG458670.20986725199682055No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAAGATCGGAAGAGCACACGTC447560.20478380383216038No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTAGATCGGAAGAGCACACGTCTG447400.2047105948576918Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGACCTCGGATCAGCCTGGATCACCCGCCGAACTAGATCGGAAGAGCAC433460.19833226295711912No Hit
GAGGTCCGGACTTGGGGAGGGTAGATCGGAAGAGCACACGTCTGAACTCC432340.19781980013583925Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCCGCGATCGTCTAGTGGTTAGGACCCTGTAGATCGGAAGAGCACACGT424460.194214258143263No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCCCGCCTAGATCGGAAGAGCACAC404420.18504483409107672No Hit
TCCCATATGGTCTAGTGGTTAGGATCCGGCAGATCGGAAGAGCACACGTC388480.17775139000964713No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCCCGCCAGATCGGAAGAGCACACG380250.1739857033854209No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCTAGATCGGAAGAGCACACG357250.1634619133055664No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAAGATCGGAAGAGCACACG354110.16202518718162104No Hit
GCGTCGGTGGTATAGTGGTGAGCATAGCTGCTAGATCGGAAGAGCACACG349130.15974655785128733No Hit
GTTCGCGCTAGTCCCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT344100.1574450507164322Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTAGATCGGAAGAGCACAC332980.15235702699086773No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCCCGCCTGTAGATCGGAAGAGCAC329830.15091572530601807No Hit
GTTCGCGCTAGTCCCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC326440.14936461015946562Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCGGTAGTATAGTGGTGAGTATCCCAGATCGGAAGAGCACACGTCTG307650.1407671312203149Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGTATAGCTCAGTGGTAGAGCATTCGACTGTAGATCGGAAGAGCACA304910.13951342753254092No Hit
TCCCATATGGTCTAGTGGTTAGGATCCGGCGCTAGATCGGAAGAGCACAC301540.13797146350779702No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC299650.13710668249688726No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA295590.13524900476974772Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGATGAAGAAATCTTATGTAAGCTAGATCGGAAGAGCACACGTCTGAA288180.13185851413967284Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCGGATGAGCTTCACTGGTGTGGAGTGCAGGCAGATCGGAAGAGCACA282990.12948379803034915No Hit
GCGTCGGTGGTATAGTGGTGAGCACAGCTGCCAGATCGGAAGAGCACACG267700.12248776540769804No Hit
TCCGGATTAGCTCAGCGGTAGAGCAGTAGATCGGAAGAGCACACGTCTGA259610.11878613663613181Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAAGATCGGAAGAGCACAC253980.11621009584701959No Hit
GCGTCGGTGGTATAGTGGTGAGCATAGCTGCCTAGATCGGAAGAGCACAC249870.11432954031535864No Hit
GCCCGGTTAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG249240.1140412799783887No Hit
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA248200.11356542164434312No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTAGATCGGAAGAGCACACGTCT244390.11182213293981069Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCGGATGAGCTTCACTGGTGTGGAGTGCAGGCTTCAGATCGGAAGAGC242090.11076975393182523No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT240910.11022983774511966Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTACGACCATACCACAGGGAAAACACCGGTTCTAGATCGGAAGAGCAC224300.10262983108310299No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)