FastQCFastQC Report
Fri 20 Dec 2024
2D2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2D2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21814155
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG10565304.843323062479386No Hit
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA4042121.8529803240143843No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3599971.6502908318016443No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA2913531.335614421003243No Hit
GGTAAAGCTTACATAAGATTTCTTCATCCATGATCGTCGGACTGTAGAAC1801820.8259866128208955No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1652910.757723597361438No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG990790.45419591086613254Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA944110.4327969614225258No Hit
CAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGA836290.38337033912154744Illumina DpnII expression Sequencing Primer (100% over 24bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT806090.3695261173306965Illumina RNA PCR Primer (100% over 50bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA792340.36322287065439846Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC787280.36090327587752086No Hit
AAAGCTTACATAAGATTTCTTCATCCATGATCGTCGGACTGTAGAACTCT750810.34418477360227795Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA739130.3388304520619753Illumina DpnII expression Sequencing Primer (100% over 25bp)
AACGCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAG704460.32293710207890247No Hit
AAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG647350.29675685352011116No Hit
AGCTTACATAAGATTTCTTCATCCATGATCGTCGGACTGTAGAACTCTGA638780.29282821177350216Illumina DpnII expression Sequencing Primer (100% over 24bp)
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC631060.28928922527597334No Hit
GGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA559110.2563060544861811No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA555040.25444029346999686No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG539850.2474769249599629No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA508750.23322012702302702No Hit
AGCGCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGA507430.2326150153421024No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT500670.22951611006706424Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAGGCGGGGATACTCACCACTATACTACCGAGGAGATCGTCGGACTGTA495000.226916880346729No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC487650.22354750848703514No Hit
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA479490.21980681809586483Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACGCAGGGTCCTAACCACTAGACGATCGCGGCGATCGTCGGACTGTAGA450710.20661354977994795No Hit
GGCGAGAATTCTACCATTGAACCACCAGTGCGATCGTCGGACTGTAGAAC439560.20150218974789535No Hit
TATCAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTC432680.19834827432004587Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT403500.1849716388280912No Hit
ACAGAATGAGTATCAAAACTACCATTATTAGAATCCTTTGATCGTCGGAC397470.18220737864932196No Hit
GGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA389060.17835208377312806No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGGAT388040.17788449747423177No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT386900.17736190102252414Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC378440.1734836852493255No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC373010.17099447583461289No Hit
AAGCTTACATAAGATTTCTTCATCCATGATCGTCGGACTGTAGAACTCTG366800.16814770042662666Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT362960.16638737553666416No Hit
AGTTCGGCGGGTGATCCAGGCTGATCCGAGGTCCGGATCGTCGGACTGTA362690.16626360269283866No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA361780.16584644236735277Illumina RNA PCR Primer (100% over 45bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG353360.16198656331175787No Hit
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC347670.15937816523262074No Hit
TAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG334450.15331788006457275No Hit
GGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTGT330010.1512825044105536No Hit
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG296740.13603093954361287No Hit
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT294530.13501783589600422No Hit
AATGAGTATCAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTA283610.13001191199017334No Hit
CATCCAAGTACTAACCCCGCCCGACAGAGCTTAACTTCGGTGATCGGACG281270.12893921401035247No Hit
GAGCGCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAG276940.1269542643297437No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC271130.12429085609779521No Hit
CAACGCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTA262090.12014675791934182No Hit
CAGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGA260500.11941787339459173No Hit
GGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTG259880.11913365427173318Illumina DpnII expression Sequencing Primer (100% over 23bp)
CATCCAAGTACTAACCCCGCCCGACAGAGCTTAACTTCGATCGTCGGACT258550.11852395841140764No Hit
GGCGGGTGATCCAGGCTGATCCGAGGTCCGGATCGTCGGACTGTAGAACT250300.11474201040562883No Hit
GCAAAGGTGTTTATGCTGCAGCCTAGATCGTCGGACTGTAGAACTCTGAA243940.11182647230662843Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAAC233460.1070222522944391No Hit
GTTCTTGGAGCCAACTTATTATGGACTTTTGGATCGTCGGACTGTAGAAC232190.10644006151051921No Hit
GATCCGGTGCATTCGGTGAGATATGGCCGTCAGCGATCGTCGGACTGTAG229940.1054086211453068No Hit
AACCAGGAATCCTAACCGCTAGACCATATGGGAGATCGTCGGACTGTAGA229430.10517482799585866No Hit
AACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGGATCGTCGGACTG228060.10454679541792933No Hit
GTATCAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACT225560.10340075056769332No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG224480.10290565919239138Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC220570.10111324504662225Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)