FastQCFastQC Report
Fri 20 Dec 2024
2D2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2D2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21814155
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA10529344.82683835335359No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC3994741.8312604820127114No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3587631.6446339544208795No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC2878841.3197119026613682No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACCAGATCGGAAGAGCACACGT1786250.8188490454936255No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1638680.7512003100738947No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA965570.4426346104169517Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC934420.4283548915830111No Hit
AAAGGATTCTAATAATGGTAGTTTTGAGATCGGAAGAGCACACGTCTGAA806200.3695765433041069Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT784830.3597801519242895No Hit
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC769450.3527296840056376Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGATGAAGAAATCTTATGTAAGCTTTAGATCGGAAGAGCACACGTCTG736360.3375606343679139Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC718920.3295658254926675Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCGTTAGATCGGAAGAGCACA691750.3171106100603026No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTAGATCGGAAGAGCACA632320.2898668318804923No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG624710.2863782713563739No Hit
ATGGATGAAGAAATCTTATGTAAGCTAGATCGGAAGAGCACACGTCTGAA623920.28601612118369935Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCCAGATCGGAAGAGCAC566110.2595149800668419No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG548200.2513047147597512No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC531190.2435070255987454No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG505490.2317256845383193No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCGCTAGATCGGAAGAGCACAC505000.23150105974767302No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG491920.22550495309123825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTGGGGGGGGGGG482310.22109955668693104TruSeq Adapter, Index 12 (97% over 41bp)
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA481640.22079241666706778No Hit
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT464390.21288470720043934Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTCGGTAGTATAGTGGTGAGTATCCCCGCCTGTAGATCGGAAGAGCAC464240.2128159445094252No Hit
GCCGCGATCGTCTAGTGGTTAGGACCCTGCGTTAGATCGGAAGAGCACAC436650.20016819354222062No Hit
GCACTGGTGGTTCAATGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT436410.200058173236598No Hit
AAAGGATTCTAATAATGGTAGTTTTGATAAGATCGGAAGAGCACACGTCT420910.19295269516513475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC395950.18151058338037848No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCAGATCGGAAGAGCACAC394120.1806716785500057No Hit
AAAGGATTCTAATAATGGTAGTTTTGATACTCATTCTGTAGATCGGAAGA393800.1805249848091755No Hit
CCTTGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGA387210.1775040105839534No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC374580.17171419200056112Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCAGATCGGAAGAGCACACGT373510.17122368480466008No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT371240.1701830760806458No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC359930.16499836917817812No Hit
ATGGATGAAGAAATCTTATGTAAGCTTAGATCGGAAGAGCACACGTCTGA357690.16397151299236665Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGACCTCGGATCAGCCTGGATCACCCGCCGAACTAGATCGGAAGAGCAC357470.1638706610455459No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA351990.16135853073382855No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT343200.15732903704039877No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAGATCGGAAGAGCA328180.15044359958018086No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAAGATCGGAAGAGCACA321870.14755098237818517No Hit
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA297680.13646185240730158No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC291930.13382594925175878No Hit
AAAGGATTCTAATAATGGTAGTTTTGATACTCATTAGATCGGAAGAGCAC279590.12816907187099386No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCGCTCAGATCGGAAGAGCACA278290.12757312854887115No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTGGGGGG270510.12400663697493669TruSeq Adapter, Index 12 (97% over 45bp)
CGTCCGATCACCGAAGTTAAGCTCTGTCGGGCGGGGTTAGTACTTGGATG269310.1234565354468234No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT267570.12265888823105914No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC256440.11755669655780845No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCGTTGAGATCGGAAGAGCAC255290.11702951592669988No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCAGATCGGAAGAGCACACGTCTGA255090.11693783233868102Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAGTTAAGCTCTGTCGGGCGGGGTTAGTACTTGGATGAGATCGGAAGAG253520.1162181161727328No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCCAGATCGGAAGAGCACACGTC248530.11393061065166173No Hit
TAGGCTGCAGCATAAACACCTTTGCAGATCGGAAGAGCACACGTCTGAAC240950.11045580266574617Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAAAAGTCCATAATAAGTTGGCTCCAAGAACAGATCGGAAGAGCACACGT232240.10646298240752392No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT230250.10555073070673607No Hit
GCTGACGGCCATATCTCACCGAATGCACCGGATCAGATCGGAAGAGCACA228820.10489519305240107No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTAGATCGGAAGAGCACAC226750.10394626791640565No Hit
CCTTGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTAGATCGGAAGAGC225930.10357036520552824No Hit
AAAGGATTCTAATAATGGTAGTTTTGATACAGATCGGAAGAGCACACGTC221480.10153040537210815No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA219190.10048062828929198Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)