FastQCFastQC Report
Fri 20 Dec 2024
2C2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2C2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20705100
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA10525975.083757141960193Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC5396002.6061211971929623No Hit
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA3091541.4931297120033227Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1877890.9069697803922704No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG1825660.8817441113542074Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT1554100.7505880193768685Illumina DpnII expression Sequencing Primer (100% over 22bp)
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG1295250.6255705116130809No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC1274600.6155971234140382No Hit
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT1129250.5453970277854248No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC945700.45674737142056787Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC763430.36871592023221333No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA755360.3648183297834833Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT748230.36137473376124724Illumina RNA PCR Primer (100% over 50bp)
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT706590.3412637466131533No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC703060.3395588526498302No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG692280.33435240592897403No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACGATCGTC687460.33202447706120713No Hit
GCTTTCAGAGTTAGAAAGAACAGAATATGAGATCGTCGGACTGTAGAACT669540.32336960459017344No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC666510.32190619702392165No Hit
GCTACTTCAGATGTAGTATCGCCAAATCGTAGATCGTCGGACTGTAGAAC616410.2977092600373821No Hit
TTTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAA584380.28223964144099767No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT579220.2797475018232223No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG524320.2532322954247987No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG492420.2378254632916528No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA488660.23600948558567697No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA481950.23276873813698074Illumina RNA PCR Primer (100% over 45bp)
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT468460.22625343514399834Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA427660.2065481451429841Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT411980.19897513173082962No Hit
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT355100.17150363920000386Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAAAGCCACGCGCCCATCTCTGTTGAGCCACCGACGCCTGATCGTCGGAC338240.1633607178907612No Hit
GCCCAGTTCAATTGTAAAAGACTACAGTCAGATCGTCGGACTGTAGAACT321830.1554351343388827No Hit
ACTGCTCGTCAGTAGTAACTCAGGCCTTGGAGATCGTCGGACTGTAGAAC318770.1539572375888066No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT316710.15296231363287305No Hit
TCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC316040.15263872186079758Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAAGCCACGCGCCCATCTCTGTTGAGCCACCGACGCCTGATCGTCGGACT311510.15045085510333203No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC310300.1498664580224196No Hit
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATTCAGAGACTCTAGTCT308670.1490792123679673No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT303530.14659673220607483Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTTCATTTGTATCCTCACGGTTGACCCCTGATCGTCGGACTGTAGAACTC299770.144780754500099Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTACAGGAGGTCCTGACCATAGATTACAGATCGTCGGACTGTAGAACTC272900.13180327552148988Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTGAATCCATGTACGATTTTCGATGTGAGATCGTCGGACTGTAGAACTC272220.13147485402147296Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCAGGTCAACAGTCCGGAAATCATGTCAAGATCGTCGGACTGTAGAACTC262700.1268769530212363Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT259120.12514791041820614Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC252830.12211001154304978Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT247210.1193957044399689No Hit
GCTTGTCAGTGGTAACTCAGGCCTTAGAGATCGTCGGACTGTAGAACTCT246040.11883062627082217Illumina DpnII expression Sequencing Primer (100% over 22bp)
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTAGATCGTCGGACTG243260.117487961903106No Hit
TTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAACT237390.11465291160148948No Hit
GGCGGGTGATCCAGGCTGATCCGAGGTCCGGATCGTCGGACTGTAGAACT229570.11087606435129509No Hit
GATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGGAC226980.109625164814466No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT225900.1091035541967921No Hit
ACTGTTCTCGCTGTTATTATATTCAACCAAGATCGTCGGACTGTAGAACT223530.10795890867467436No Hit
GACAAGGAAATGCACAGTTACACGTGAGATCGTCGGACTGTAGAACTCTG222850.10763048717465747Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTTTTTTCAAGAATCCATCCCGCCTATCAATCACTGATCGTCGGACTGTA218760.1056551284466146No Hit
ACTTGGTTGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT215660.10415791278477285No Hit
TTTTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGA213440.10308571318177646No Hit
TAAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTG212120.10244818909350836Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT211000.10190725956406876Illumina DpnII expression Sequencing Primer (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)