FastQCFastQC Report
Fri 20 Dec 2024
2C2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2C2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20705100
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC10422025.03355212000908Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT5487502.6503132078570015No Hit
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC3065461.4805337815320863Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1894710.9150933827897475No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA1808530.8734707873905463Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG1556480.7517374946269276Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA1291360.6236917474438666No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT1266860.6118589139873751No Hit
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC1126210.5439287904912317No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT939690.4538447049277714Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT766910.37039666555582923No Hit
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC745330.359974112658234Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT706800.3413651708999232No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC700570.3383562503924154No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC686610.331613950186186No Hit
GTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGG680240.32853741348749826No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGCAGATCGGAAGAGCACACGTC668640.3229349290754452No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT665690.32151015933272475No Hit
TACGATTTGGCGATACTACATCTGAAGTAGCAGATCGGAAGAGCACACGT622790.3007906264640113No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAAGATCGGAAGAGCACACG586820.28341809505870535No Hit
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA576300.27833722126432614No Hit
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA522010.25211662827032955No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA489460.23639586382099095No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG488910.2361302287842126No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG472170.22804526421026702Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA431920.20860560924603117Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC410360.19819271580431874No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG352800.1703928017734761Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGTCGGTGGCTCAACAGAGATGGGCGCGTGGCTTTTAGATCGGAAGA328170.1584971818537462No Hit
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAA328160.15849235212580476No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGTAGATCGGAAGAGCACACGT322870.1559374260447909No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGCAGATCGGAAGAGCACACGTC320440.1547638021550246No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC315240.15225234362548357No Hit
TGAAAATCTTTCTTCTGAGGCGGAAGATCGGAAGAGCACACGTCTGAACT311500.15044602537539062Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT308350.14892466107384172No Hit
AGGCGTCGGTGGCTCAACAGAGATGGGCGCGTGGCTTTAGATCGGAAGAG306240.147905588478201No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC301240.14549072450748848Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGGTCAACCGTGAGGATACAAATGAAAAGATCGGAAGAGCACACGTCT298500.14416737905153804Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACATCGAAAATCGTACATGGATTCACTAGATCGGAAGAGCACACGTCT271450.13110296496998325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTAATCTATGGTCAGGACCTCCTGTAGTAGATCGGAAGAGCACACGTCT270000.1304026544184766Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGACATGATTTCCGGACTGTTGACCTGGAGATCGGAAGAGCACACGTCT260090.12561639402852437Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG256940.12409502972697548Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT251220.12133242534448035Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC247840.11969997730027868No Hit
TCTAAGGCCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG246300.11895619919729923Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGC242110.11693254318984213No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAGATCGGAAGAGCACACGTC235550.11376424166026729No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCCAGATCGGAAGAGCACACGTC232250.112170431439597No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCAGATCGGAAGA227720.10998256468213147No Hit
TCACGTGTAACTGTGCATTTCCTTGTCAGATCGGAAGAGCACACGTCTGA224710.10852881657176251Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC224310.10833562745410552No Hit
TTGGTTGAATATAATAACAGCGAGAACAGTAGATCGGAAGAGCACACGTC221690.10707023873345214No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAAAGATCGGAAGAGCACAC221050.10676113614520094No Hit
AGTGATTGATAGGCGGGATGGATTCTTGAAAAAAAAGATCGGAAGAGCAC216550.10458775857155966No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGTAGATCGGAAGAGCACACGTC214220.10346243196120762No Hit
CACCCCTCCCCGCACATTGACCTCTTAAGATCGGAAGAGCACACGTCTGA211840.10231295671114846Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG211510.10215357568908144Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAAGGCCTGAGTTACTACTGACGAGCAGATCGGAAGAGCACACGTCTG208280.10059357356400114Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)