FastQCFastQC Report
Fri 20 Dec 2024
2C1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2C1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21593184
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC9228164.273644868677079No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT4492282.0804157460057766No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG3712651.7193619986751376Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA2572081.1911536529304803No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1709460.7916664814230269No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC1270760.5885005194231662No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC1183970.5483072806678255No Hit
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC979020.4533930707023105No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC854210.39559242397971506No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT847250.39236918464641435Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT845500.39155874372209304No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA817310.37850369820402585Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT686170.31777157088088537Illumina RNA PCR Primer (100% over 50bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT659210.30528614955534117No Hit
CATCCAAGTACTAACCCCGCCCGACAGAGCTTAACTTCGGTGATCGGACG626620.2901934239989804No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGGAT624720.2893135167097173No Hit
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC604400.27990313980559794No Hit
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA563730.26106849272437077Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG506260.23445361276965915No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCAGATCGTCGGACTG505410.23405997003498885No Hit
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA497690.2304847677859828No Hit
GGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTG493390.22849339865765048Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC474940.21994903576980587No Hit
AACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGGATCGTCGGACTG436330.20206839343377986No Hit
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT427000.19774758553439825Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT425420.19701587315701102Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT422700.1957562164060659No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC415510.19242646197985439Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG412370.19097229940707217Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGTTCGGCGGGTGATCCAGGCTGATCCGAGGTCCGGATCGTCGGACTGT395830.18331247489948682No Hit
AACTGAAATTAAGAGTGGATAACCCTCCCCAGGTCCGGACCTCGATCGTC382460.17712070623767204No Hit
GATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGA374150.17327226961989486Illumina DpnII expression Sequencing Primer (100% over 24bp)
AAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACTC374010.17320743434594915Illumina DpnII expression Sequencing Primer (100% over 21bp)
CATCCAAGTACTAACCCCGCCCGACAGAGCTTAACTTCGATCGTCGGACT365670.16934510445518364No Hit
GGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG365300.16917375408832713Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAACCGGTGTTTTCCCTGTGGTATGGTCGTAGACGATCGTCGGACTGTA338830.15691525622159289No Hit
AGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAA322600.1493989955348873No Hit
CATCCAAGTACTAACCCCGCCCGACAGAGCTTAACTTCGGTGATCGATCG321390.14883863352435658No Hit
AGTTCGGCGGGTGATCCAGGCTGATCCGAGGTCCGGATCGTCGGACTGTA321360.14882474025136821No Hit
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA311820.14440667944106805No Hit
GGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA311470.1442445912562038Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT305510.14148446102251525No Hit
TAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG293780.13605219128406446No Hit
AGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAACTC291340.13492220508101074Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC283880.13146741119790395No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA283510.13129606083104742Illumina RNA PCR Primer (100% over 45bp)
GCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTAG275410.12754487712418883Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA269250.12469212507057782No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT262770.12169117810509095No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGTAATCGATCGTCGGACTGTAGAA260610.12069086244992865No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA255560.11835216149688717No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT253750.11751393402658913Illumina DpnII expression Sequencing Primer (100% over 22bp)
TAAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACT249270.11543920526032658No Hit
AGCCGAGATATCCGTCGCTGAAAGTTGTGGATCGTCGGACTGTAGAACTC247740.11473064833791996Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCT239260.11080348317320872Illumina DpnII expression Sequencing Primer (100% over 22bp)
GATCAAGTTCGACCGGCTTTCCCGAACACGACAAGCGATCGTCGGACTGT239220.11078495880922423No Hit
GGCGGGTGATCCAGGCTGATCCGAGGTCCGGATCGTCGGACTGTAGAACT238720.11055340425941815No Hit
ACTCTGCGGCGTACAATACCTCGTTCTCTTGATCGTCGGACTGTAGAACT233610.10818691676039996No Hit
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA233490.10813134366844648Illumina DpnII expression Sequencing Primer (100% over 25bp)
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG223370.10344467958037129No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG222270.1029352595707979No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)