FastQCFastQC Report
Fri 20 Dec 2024
2C1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2C1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21593184
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT9400474.353443197631252No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC4547222.105858959938469No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA3689941.708844791022945Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC2574561.1923021634975184No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1724160.7984741851873258No Hit
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA1264420.5855644077316249No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG1173880.5436345098527388No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCAGATCGGAAGAGCACACGT984810.456074472389065No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT861280.39886660531397317No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG851160.39417994122589795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC849120.3932351986626891No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC812120.37610016197703866Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC658030.3047396808177988No Hit
CCTTGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGA622300.28819279268865583No Hit
CGTCCGATCACCGAAGTTAAGCTCTGTCGGGCGGGGTTAGTACTTGGATG614730.28468705680459166No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT596510.2762492090096579No Hit
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT558290.2585491792224806Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC503310.23308744092580325No Hit
TGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGC502460.2326937981911329No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAGATCGGAAGAGCACAC498460.23084136179268422No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCAGATCGGAAGAGCACACGTCTGA495850.22963264704269643Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT478060.22139393616059586No Hit
CCTTGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTAGATCGGAAGAGC437360.2025453958063804No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG425490.19704829079398387Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC424910.1967796875162088No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC422880.1958395760439961Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA415880.1925978123467109Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT415450.19239867543387765Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACCTCGGATCAGCCTGGATCACCCGCCGAACTTAGATCGGAAGAGCA394910.1828864145278436No Hit
GAGGTCCGGACTTGGGGAGGGTTATCAGATCGGAAGAGCACACGTCTGAA375230.173772427447476Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGTCCGGACCTGGGGAGGGTTATCCACTCTTAATTTCAGTTAGATCGG375160.17374000981050317No Hit
TACCACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCT374270.17332784271184834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGTTTGGTCTAGTGGTATGATTCCAGATCGGAAGAGCACACGTCTGA370650.17165138777125227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAGTTAAGCTCTGTCGGGCGGGGTTAGTACTTGGATGAGATCGGAAGAG360790.16708513204907624No Hit
GTCTACGACCATACCACAGGGAAAACACCGGTTCTAGATCGGAAGAGCAC339320.15714217968040284No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTAGATCGGAAGAGCACACG322800.14949161735480973No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCCGAACTAGATCGGAAGAGCAC321010.14866265206650395No Hit
GATCACCGAAGTTAAGCTCTGTCGGGCGGGGTTAGTACTTGGATGAGATC318580.14753729695444637No Hit
GTTCGCGCTAGTCCCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT313500.14518470272841652Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC312810.14486515744968412No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC307330.1423273195838094No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAGATCGGAAGAGCACA293210.1357882190972855No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTAGATCGGAAGAGCACACGTCT290190.13438962961645676Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT284250.13163876156476043No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTCAGATCGGAAGAGCAC273780.12679000929182097No Hit
GTTCGCGCTAGTCCCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC273440.12663255219795283Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC260920.12083442627080841No Hit
GATTACCACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACG258800.11985263497963061No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG256830.11894031005339463No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGGGGGGGGG256010.11856056059171266TruSeq Adapter, Index 3 (97% over 41bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG254350.11779179948635643Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACAACTTTCAGCGACGGATATCTCGGCTAGATCGGAAGAGCACACGTCT249390.11549477835228006Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCACCCCTCCCCGCACATTGACCTCTTAAGATCGGAAGAGCACACGTC248480.11507334907163297No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCCAGATCGGAAGAGCACACGTC242300.11221133483602973No Hit
GCTTGTCGTGTTCGGGAAAGCCGGTCGAACTTGATCAGATCGGAAGAGCA240100.11119249481688295No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCAAGATCGGAAGAGCACACGTCTG238100.1102662766176586Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGAACGAGGTATTGTACGCCGCAGAGTAGATCGGAAGAGCACACGTC232670.1077515942067645No Hit
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC231510.1072143876512144Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA224740.10407913904683996No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGGGG217920.10092073498748494TruSeq Adapter, Index 3 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)