FastQCFastQC Report
Fri 20 Dec 2024
2B3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2B3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26026014
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC14975655.754108178071371No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA10023633.851388845022522Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4724171.8151723118261596Illumina RNA PCR Primer (100% over 45bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT4038041.5515399323154133No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3294641.2659026464828613No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT2634031.0120758407338135Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT2544830.9778024402814814Illumina RNA PCR Primer (100% over 50bp)
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG2292590.8808840262669497No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG2058850.7910738847677559Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2028970.7795930640781181Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG1678740.6450238595891019No Hit
GACGGAGACTCCTTGCATGTATTAGCTCTAGAATTACTACGGTTATCCAT1621750.6231265379323934No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC1156810.44448220153881424No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1075950.41341328718258585No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA971590.37331494557714445No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC954310.3666754348168721Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG928400.3567200109859312No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT900930.34616518687802134No Hit
GCTACTTCAGATGTAGTATCGCCAAATCGTAGATCGTCGGACTGTAGAAC867350.33326271168531607No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC848150.3258854775072356No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT830880.319249809056431Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC792830.30462982153164136No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACGATCGTC786430.30217074347228123No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT766940.294682082319636No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA754990.2900905225056745Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG746810.28694751336105484No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT727670.2795933330397809Illumina DpnII expression Sequencing Primer (100% over 22bp)
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT653790.2512063506920422No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT615000.23630203226663904Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGGAGACTCCTTGCATGTATTAGCTCTAGAATTACTACGGTTATCCATG572330.21990689776774883No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT554390.21301379458260492No Hit
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT546190.20986310081904974No Hit
ACTGCTCGTCAGTAGTAACTCAGGCCTTGGAGATCGTCGGACTGTAGAAC513120.19715658340919975No Hit
GATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGGAC501120.19254581204789945No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA438860.16862359330168653Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCTTTCAGAGTTAGAAAGAACAGAATATGAGATCGTCGGACTGTAGAACT426030.163693910254563No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT424900.1632597292847072No Hit
AAAAGCCACGCGCCCATCTCTGTTGAGCCACCGACGCCTGATCGTCGGAC414140.15912540429740796No Hit
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATTCAGAGACTCTAGTCT412990.15868353870861668No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG409240.15724267265821035No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC390740.15013440014287244No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT381280.14649957538638073Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA371340.142680319775437No Hit
GCTTGTCAGTGGTAACTCAGGCCTTAGAGATCGTCGGACTGTAGAACTCT362500.1392837182059458Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAAGCCACGCGCCCATCTCTGTTGAGCCACCGACGCCTGATCGTCGGACT361750.13899554499586453No Hit
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT356460.13696296328742466Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC352770.1355451510938248Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT352380.13539530102458255Illumina DpnII expression Sequencing Primer (100% over 22bp)
GATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCGGA323370.12424876125863914No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT320210.12303459146683009No Hit
TAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG304380.11695221557938146No Hit
TATCAGACTAACATGAGATATCAGCATCAAAGCTAAGACAGGGGTAACTG296860.11406279885963327No Hit
TCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC293320.1127026213080497Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT290260.11152687461091813No Hit
GCTCGTCAGTAGTAACTCAGGCCTTGGAGATCGTCGGACTGTAGAACTCT283350.10887183876870273Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAAC282810.10866435405744422No Hit
TTTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAA282620.10859135017755696No Hit
GATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGA278880.10715432643661837Illumina DpnII expression Sequencing Primer (100% over 24bp)
TTTCATTTGTATCCTCACGGTTGACCCCTGATCGTCGGACTGTAGAACTC276440.10621680292648732Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTTGGTTGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT270650.10399210574465995No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT265980.10219774722322059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)