FastQCFastQC Report
Fri 20 Dec 2024
2B3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2B3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26026014
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT14270675.4832330452139155No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC9332303.585758464588546Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC3819321.4675009396367804No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTGGGGGG3288141.2634051453288238TruSeq Adapter, Index 18 (97% over 45bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT3210671.2336387738821626No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG2457600.9442859747942962Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA2283880.8775373747205393No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA1928360.740935588523083Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC1891660.7268343127764397Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC1590030.6109387323006896No Hit
CACTACATGGATAACCGTAGTAATTCTAGAGCTAATACATGCAAGGAGTC1272010.4887456066072968No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTGGGGGGGGGGG1235870.47485950019084755TruSeq Adapter, Index 18 (97% over 41bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT1090580.4190345859339044No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1044660.40139070085799544No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG929080.3569812880297383No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA906490.3483015109420905No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT895340.344017335885549Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC873000.33543361653459497No Hit
TACGATTTGGCGATACTACATCTGAAGTAGCAGATCGGAAGAGCACACGT817690.3141818028684685No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTAGGGGG812830.31231444046714185TruSeq Adapter, Index 18 (97% over 45bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT799780.3073002266117278No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG774660.29764834522873923Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGG769930.29583093285049333No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT754040.28972550310623824No Hit
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA736530.2829976192282076No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC725970.27894014043026333No Hit
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC698600.26842373941703096Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG684370.2629561330444224Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTACATGGATAACCGTAGTAATTCTAGAGCTAATACATGCAAGGAGTCT621540.23881490265854774No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC604070.2321023880183881No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG573260.22026423254824962Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC518930.1993889652099626No Hit
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC517460.19882414571820334No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGTAGATCGGAAGAGCACACGT495000.19019431865363634No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCAGATCGGAAGA485710.18662481315809637No Hit
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAA444970.17097124438648192No Hit
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA418280.16071612041705657Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA408560.15698139561440336No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGCAGATCGGAAGAGCACACGTC396980.1525320012507486No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG382560.14699139099825276No Hit
AGGCGTCGGTGGCTCAACAGAGATGGGCGCGTGGCTTTTAGATCGGAAGA376110.14451310139155385No Hit
GCACTACATGGATAACCGTAGTAATTCTAGAGCTAATACATGCAAGGAGT368140.1414507807457569No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAGATCGGAAGAGCACAC354360.13615607829919713No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC344620.1324136688776084Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCAAGACCTGAGTTACCACTGACAAGTAGATCGGAAGAGCACACGTCTG337360.1296241522040217Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTAAGGCCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG336180.12917075968682717Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT331280.12728802804762956Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG330240.12688842786298354Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGTCGGTGGCTCAACAGAGATGGGCGCGTGGCTTTAGATCGGAAGAG327760.12593553511498148No Hit
CTACATGGATAACCGTAGTAATTCTAGAGCTAATACATGCAAGGAGTCTC315840.12135550222942323No Hit
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCAGATCGGAAG315020.1210404328530677No Hit
AGTGGTGATGATATCCCAGTTACCCCTGTCTTAGCTTTGATGCTGATATC303380.11656798463260644No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC302200.1161145921154119No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAGATCGGAAGAGCACA287530.11047792412622233No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC281340.10809953456568493No Hit
TGAAAATCTTTCTTCTGAGGCGGAAGATCGGAAGAGCACACGTCTGAACT270050.10376156717659493Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAAGATCGGAAGAGCACACG269540.10356560939373967No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT269370.1035002901327879No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGATCGGAAGAGCACACGTCTG267980.10296620911677062Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGTCAACCGTGAGGATACAAATGAAAAGATCGGAAGAGCACACGTCT260880.10023816939466798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)