FastQCFastQC Report
Fri 20 Dec 2024
2B2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2B2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19288036
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC5999763.110612195041527No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT4654162.4129776613855345Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT2140321.1096619686939613No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG1994451.0340347767911673Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCAGATCGTCGGACTG1740550.9023987719641337No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG1711410.8872909610911137Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGATC1145120.5936944539091487No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1067380.553389676377626No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA926850.48053104007064273Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATGATCGTCGGACT846090.4386605251047852No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG689670.3575636213039005No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA661110.34275651497124954No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC609740.316123424904433No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC562010.2913775150564837No Hit
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC472950.24520381442672545No Hit
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT465390.24128428627984724No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT453140.23493319900481313Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT440630.22844731314271707Illumina RNA PCR Primer (100% over 50bp)
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC417660.216538376431898No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT387820.20106764628601898No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGGAT331220.17172303079484091No Hit
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA317520.16462018216888438No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC307020.15917639307599799Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGTTA302670.15692110902323078No Hit
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT284400.14744891600160848Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA278170.14421893447316253Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG276110.14315091489874862No Hit
ACCGCTCAGAATGCGATCACCTCCTCCGGATCGTCGGACTGTAGAACTCT272000.14102006031096168Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCCGATCGTCGGA264660.13721459250698204No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC245810.12744169494499077No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG244690.12686102410841624No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA243490.12623887678351492Illumina RNA PCR Primer (100% over 45bp)
TGCTCAGGAAGAAATACAGAGTACCGATGATCGTCGGACTGTAGAACTCT240280.12457463268940394Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA236750.12274448264198595No Hit
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC234560.12160906377404108No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG228980.11871607871325No Hit
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT219770.11394109799463252No Hit
GGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG211380.10959125128136425Illumina DpnII expression Sequencing Primer (100% over 23bp)
GATCAAGTTCGACCGGCTTTCCCGAACACGACAAGCGATCGTCGGACTGT208780.10824326541074478No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT206020.10681232656347178No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT202410.10494070002772704Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT197250.10226546653065144No Hit
GAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCAGATCGTCGGACTG195680.10145149044723889No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)