FastQCFastQC Report
Fri 20 Dec 2024
2B2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2B2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19288036
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT6086273.1554638326058706No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG4653932.412858416481595Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2160071.1199014767496285No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA1976031.0244848153539323Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGC1725570.894632299524949No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA1709430.8862644180050265Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGAT1138640.5903348583546816No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1073060.5563345070488255No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC918600.4762537772119463Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAG842250.43666965366510097No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC683640.35443733099627145No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC659220.34177663293453No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT610430.31648115961625123No Hit
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA558040.2893192443232686No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCAGATCGGAAGAGCACACGT472370.2449031098863565No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC469010.24316109737663288No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC447000.2317498785257348Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG413730.21450084394284621No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC388550.20144611924200057No Hit
CCTTGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGA330000.17109051434785794No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC316620.16415357167520842No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT305800.158543876629015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG282230.14632386625574526Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA275820.14300056262856414No Hit
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT273670.14188588200478267Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGAGGAGGTGATCGCATTCTGAGCGGTAGATCGGAAGAGCACACGTCTG269250.13959430602472953Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAG262680.13618804942089488No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT245500.1272809735527246No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA243900.12645144378618953No Hit
ATCGGTACTCTGTATTTCTTCCTGAGCAAGATCGGAAGAGCACACGTCTG239030.12392656255929842Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG236680.12270819071470004No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT232100.1203336617579934No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA231460.12000184985137938No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC218870.11347448750095655No Hit
GGCTGTTTGGTCTAGTGGTATGATTCCAGATCGGAAGAGCACACGTCTGA213570.11072667014930915Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTGTCGTGTTCGGGAAAGCCGGTCGAACTTGATCAGATCGGAAGAGCA208410.10805143665223353No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC205330.10645459185165353No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG200430.1039141569416399Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTCAGATCGGAAGAGC197890.10259727843726547No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC197280.10228102021377398No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)