FastQCFastQC Report
Fri 20 Dec 2024
1H8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9570686
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC5820506.081591225540155No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2400472.5081483187307576No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1010971.0563192648886401No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1000571.0454527501999333Illumina RNA PCR Primer (100% over 50bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC739820.77300624009606No Hit
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA545070.5695203039782102Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA478440.4999014699677745Illumina RNA PCR Primer (100% over 45bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA459220.4798193149372991No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA455430.47585930621901085Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT425140.44421058218815246No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG403710.4218192927863269Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT397190.4150068239622531Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA375700.39255284312953115No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG352890.36871965081708874No Hit
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT340340.3556066931879282No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT300950.3144497688044514No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT299420.31285113731659364Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT292450.30556848276079684No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC262980.27477654161885573Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT252330.2636488126347474Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT210060.2194826995682441No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA197390.20624435907729083Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACTCGTCGTATGTCCCTTAAATTCCATAAGATCGTCGGACTGTAGAACTC193630.20231569607445068Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA179400.18744737838019135No Hit
CAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGA177460.18542035544787488Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT169740.17735405800587337Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTGAATCCATGTACGATTTTCGATGTGAGATCGTCGGACTGTAGAACTC168740.17630920082426693Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTTGGTCGACAGGTTCTGCACATAAAAGAGATCGTCGGACTGTAGAACT167910.17544196936353362No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC165080.17248502353958745No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC165030.17243278068050713No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC163510.17084459776446537No Hit
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA154540.1614722288454558Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT141670.1480249169181812No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131670.13757634510211703No Hit
GCTCCAGATTGAGACAACAGATACGTAAGATCGTCGGACTGTAGAACTCT131370.1372628879476351Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT129960.13578963932157007No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT129170.13496420214810098No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT124820.13041907340811307Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCGGATCCTAACCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT113100.11817334723968585No Hit
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA112480.11752553578708988Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG108950.11383718993601923No Hit
ACTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT103880.10853976402527468Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT101830.10639780680298153No Hit
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC98010.10240645236924499No Hit
GCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTAG97200.10156011805214382Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA95950.1002540465751358No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)