FastQCFastQC Report
Fri 20 Dec 2024
1H8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9570686
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT5443355.687523339497294No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT2317142.4210803697874947No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC941570.9838061764851547No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT714770.7468325676968192No Hit
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC494010.5161698962853865Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC437900.4575429598254503No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC415930.4345874475455574Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC409190.4275451101415301No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA372550.3892615430074709Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG362090.37833233688786777Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG356170.37214678237275783No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC333130.34807327290854595No Hit
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC320760.33514838957207455No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC276470.2888716649987263No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC274910.2872416877954203No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG273550.2858206820284356Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT243820.2547570780192768Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC226030.2361690687584986Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC190390.19893035880604587No Hit
TTATGGAATTTAAGGGACATACGACGAGTAGATCGGAAGAGCACACGTCT180960.18907735558349736Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC179680.18773993839104114Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAGATCGGAAGAGCACAC169530.177134637997736No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTGGGGGG167520.17503447506270708TruSeq Adapter, Index 16 (97% over 43bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG159710.16687414047436097No Hit
TCACATCGAAAATCGTACATGGATTCACTAGATCGGAAGAGCACACGTCT159430.16658158046351118Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGGATTCTAATAATGGTAGTTTTGAGATCGGAAGAGCACACGTCTGAA157510.16457545467482684Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTTTATGTGCAGAACCTGTCGACCAAGTAGATCGGAAGAGCACACGTC157290.16434558609487343No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT155580.1625588803143265No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG155530.16250663745524616Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT152480.1593198230513466No Hit
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC141740.14809805692089364Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTAGGGGG139290.14553815682595794TruSeq Adapter, Index 16 (97% over 43bp)
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC132920.13888241657912503No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTGGGGGGGGGGG132480.1384226794192182TruSeq Adapter, Index 16 (97% over 39bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC124560.13014741054089538No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC121650.12710687614242072No Hit
TTACGTATCTGTTGTCTCAATCTGGAGCAGATCGGAAGAGCACACGTCTG118060.12335583886045369Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG113540.11863308439959268Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCATATGGTCTAGTGGTTAGGATCCGGCAGATCGGAAGAGCACACGTC106110.11086979554025699No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAGATCGGAAGAGCACA102000.10657543252385461No Hit
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT101000.1055305753422482Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCAAGACCTGAGTTACCACTGACAAGTAGATCGGAAGAGCACACGTCTG98910.10334682383269078Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC97880.10227062093563616No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)