FastQCFastQC Report
Fri 20 Dec 2024
1H7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9834424
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2598422.6421679602181074No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT897930.9130478816044538No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG706030.7179169822248868Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA632150.6427931112183083Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC598470.608546062280821No Hit
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT596450.6064920528136676No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT474370.48235666877897476Illumina DpnII expression Sequencing Primer (100% over 22bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA351040.3569502392819346Illumina RNA PCR Primer (100% over 45bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT349400.3552826276353348Illumina RNA PCR Primer (100% over 50bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT344660.35046282324211364No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT328960.33449849223503075Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC316040.3213609663362084Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT291820.2967331894577659Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG284180.2889645595918988No Hit
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA250840.25506323502017Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC234090.2380312258247153No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC227580.23141162105681026No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC215300.21892487043470976No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT206170.20964115437772463No Hit
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA205880.20934627183046Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT191750.19497837392408543No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT177620.18061047601771085Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGA174230.1771634007238248Illumina DpnII expression Sequencing Primer (100% over 24bp)
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA173240.17615673271764568Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGACAGCACAGTCAATTGATTTCTTCGTTAGATCGTCGGACTGTAGAACT172490.17539410544023729No Hit
GATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGA166650.16945578104015038Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA162010.16473766028391698No Hit
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA158560.16122957480783826No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT137520.13983533758560746Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC137210.13952011831094532No Hit
AGTGAATCCATGTACGATTTTCGATGTGAGATCGTCGGACTGTAGAACTC135220.13749661393488832Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT133300.13554428810472274No Hit
TAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG127880.13003303497998459No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA126640.128772157881336No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC125870.12798919387653004No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG123930.12601653131896692Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCTGGATGATCAGATCCACCAGTCGCTGGATCGTCGGACTGTAGAACTC122170.12422689930798184Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT116290.11824790145309985No Hit
ACTGTTCTCGCTGTTATTATATTCAACCAAGATCGTCGGACTGTAGAACT110350.11220789341602519No Hit
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC106060.10784566538924903Illumina DpnII expression Sequencing Primer (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)