FastQCFastQC Report
Fri 20 Dec 2024
1H7_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H7_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9834424
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2640462.684915761207774No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC906800.9220672202052707No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA696390.708114679619264Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC622700.6331840075229622Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT600340.6104475462924925No Hit
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC594930.6049464615314532No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG476060.4840751222440684Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC342780.3485511708667432No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC324730.33019727439044727Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT315290.3205983390588Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG287700.292543823613869Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC280830.2855581577528079No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGGG269200.27373235077112806TruSeq Adapter, Index 8 (97% over 45bp)
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC247260.2514229608160071Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT231650.23555014508221325No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG224850.22863565776704362No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT214880.21849779915936104No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC205920.20938694528525514No Hit
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC202860.2062754259934288Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC193820.19708322520973265No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG178470.18147478693210706Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACGAAGAAATCAATTGACTGTGCTGTCCAGATCGGAAGAGCACACGTC174700.1776413138176674No Hit
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC171040.17391969270391433Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAGGATTCTAATAATGGTAGTTTTGAGATCGGAAGAGCACACGTCTGAA170670.17354346324705952Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGTCCGGACTTGGGGAGGGTTATCAGATCGGAAGAGCACACGTCTGAA166100.16889652103671754Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG161750.16447328282774873No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAGATCGGAAGAGCACAC157310.1599585293454909No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG137920.1402420721335586Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT136690.1389913633986088No Hit
TCACATCGAAAATCGTACATGGATTCACTAGATCGGAAGAGCACACGTCT134890.1371610579328286Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC131650.13386650809442424No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAGATCGGAAGAGCACA126930.1290670404286006No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC126060.12818239278680682No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT125680.12779599496625324No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA122970.12504036840388416Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGCGACTGGTGGATCTGATCATCCAGCAAGATCGGAAGAGCACACGTCT122260.12431841458127085Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC115980.11793268217843769No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGGGGGGGG115510.1174547690845951TruSeq Adapter, Index 8 (97% over 41bp)
TTGGTTGAATATAATAACAGCGAGAACAGTAGATCGGAAGAGCACACGTC109290.11113004686395461No Hit
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT105070.10683899738306991Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)