FastQCFastQC Report
Fri 20 Dec 2024
1H6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18878768
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA7694194.075578448763182Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC4467912.3666321870155933No Hit
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2455361.3005933438029431Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC1666790.882891298838992Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1658420.8784577468190721Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG1537880.8146082413852429No Hit
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT1531450.8112022987940738No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1466320.7767032255494638No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT1414720.7493709335270182Illumina DpnII expression Sequencing Primer (100% over 22bp)
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG1310310.6940654178281125No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG1145760.60690400983793No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC1101920.583682155530488No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG807150.42754378887435873Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG698440.3699605821735825No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA668630.3541703568792201Illumina RNA PCR Primer (100% over 45bp)
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT624450.33076840607395563Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC597650.31657256448090254No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA572720.3033672536258722Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC566430.30003546841615936Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT550160.2914173212997797No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGGATCGTCGGACT545920.2891714120328191No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT518280.2745306261510285Illumina RNA PCR Primer (100% over 50bp)
CATCAAGTTTTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG503330.26661167720266493Illumina RNA PCR Primer (100% over 33bp)
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATTCAGAGACTCTAGTCT499620.26464650659407435No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT457140.24214503827792153No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT423760.22446379975642478No Hit
GCTTTCAGAGTTAGAAAGAACAGAATATGAGATCGTCGGACTGTAGAACT421270.22314485775766724No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACGATCGTC373190.19767709418326448No Hit
TTTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAA353270.18712555819320412No Hit
GCTCCAGATTGAGACAACAGATACGTAAGATCGTCGGACTGTAGAACTCT326130.1727496201023287Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC309080.16371831043212143Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC302310.16013227134313002No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG297210.1574308238757953Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAACG289130.15315088357460613Illumina Small RNA Adapter 1 (100% over 26bp)
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG287840.152467576274045Illumina RNA PCR Primer (100% over 33bp)
ACACGCTTTGTCCATAACAAGAGACCATAGATCGTCGGACTGTAGAACTC272630.14441090647440552Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACTC271270.14369052048311626Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT270920.14350512702947565Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTCGTCGTATGTCCCTTAAATTCCATAAGATCGTCGGACTGTAGAACTC269960.14299661927091853Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTCAGAATAAACAATTTTCTCAATCCTACCAAAATCAGATCGTCGGACT268880.14242454804254176No Hit
TTCCGCCTCAGAAGAAAGATTTCCAGATCGTCGGACTGTAGAACTCTGAA268830.14239806326345023Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCTATTCAGAGAATTAAGACATATCGTAGATCGTCGGACTGTAGAACTC260440.1379539173318937Illumina DpnII expression Sequencing Primer (100% over 21bp)
TATCATTTGTGTTCTTGACAAGTGGAATCAGATCGTCGGACTGTAGAACT248750.131761775980297No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT246980.13082421480045733Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGATCGTCGGAC244070.12928280065733103No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT240260.12726466049055743No Hit
GATTTTCCTCAGGTTGAGCTTCGTCTTCAGATCGTCGGACTGTAGAACTC238480.12632180235489943Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTCAGATAAAGGCATCTCAACACGTAAAGATCGTCGGACTGTAGAACTC237860.1259933910941646Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC237460.1257815128614325No Hit
TTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAACT223820.11855646512526664No Hit
TTTCATTTGTATCCTCACGGTTAACCCCTGATCGTCGGACTGTAGAACTC223660.11847171383217381Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTCATTTGTATCCTCACGGTTGACCCCTGATCGTCGGACTGTAGAACTC221720.11744410440342294Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGAGTCTTTAGGCTTAAATCGTTGATACGATCGTCGGACTGTAGAACTC214600.11367267186079091Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT214120.11341841798151236Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTGAATATGTACTTCGAAGTTCCATGAGAGATCGTCGGACTGTAGAACT213550.11311649149986906No Hit
TTTCAGAAATGATATTGGCCTATCACCACGTCTGATCGTCGGACTGTAGA213260.11296287978113827No Hit
GGTCTTCCTTGAGTGTATCACGTCTAGTAGATCGTCGGACTGTAGAACTC213050.11285164370895388Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTTTTTTTCCATCGCGAGTAAACAATTAAGATCGTCGGACTGTAGAACT212320.11246496593421773No Hit
ACTTGGTTGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT207160.10973173673197319No Hit
ACTTTTCGAATTTTTCAAAGGTAGATGTAAGATCGTCGGACTGTAGAACT202490.1072580583648255No Hit
TAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG202210.10710974360191301No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC197160.10443478091366978No Hit
ACAGACCCAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG192020.10171214562306183No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)