FastQCFastQC Report
Fri 20 Dec 2024
1H6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18878768
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC7251493.84108221468689Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4391552.3261846323870286No Hit
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC2321181.229518790632948Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT1582160.8380631617486903Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC1556960.8247148330865659Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC1500640.7948823779178811No Hit
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC1468460.7778367740945807No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1423810.754185866365856No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG1347090.7135476213278323Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA1300770.6890121219774511No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA1135910.6016865083569013No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT1058830.5608575729094186No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA765360.40540781050966884Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA695190.3682390715326339No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG599420.31751012566074227Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT578630.3064977545144895No Hit
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA553110.2929799232661792Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTGGGGGG545100.2887370616557182TruSeq Adapter, Index 10 (97% over 36bp)
CGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAG536330.28409163140306615No Hit
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT535560.2836837658050568Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC524800.2779842413445623No Hit
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAA523790.2774492488069137No Hit
TAAAACTTGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGG461020.24420025713542326TruSeq Adapter, Index 10 (97% over 36bp)
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA446280.23639254425924403No Hit
TATGATTGAAATCTTCATGAACGACACAAAAGGCCAAAAAAAAAAAAAAA405540.21481274625547597No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC404030.2140129059269122No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGCAGATCGGAAGAGCACACGTC400610.2122013470370524No Hit
GTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGG369050.19548415447448692No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAAGATCGGAAGAGCACACG343420.18190805671217528No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTGGGGGGGGGGG314980.16684351436492043TruSeq Adapter, Index 10 (96% over 32bp)
TTACGTATCTGTTGTCTCAATCTGGAGCAGATCGGAAGAGCACACGTCTG308830.16358588653666384Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG297420.15754205994797965No Hit
TGAAAATCTTTCTTCTGAGGCGGAAGATCGGAAGAGCACACGTCTGAACT289040.15310321097224142Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA285470.1512121977451071Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAACGAGTAGATAAATGAAGAGAGATCGGAAGAGCACACGTCTGAACTC270900.14349453311783905Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGGTCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGG266280.14104733952978288TruSeq Adapter, Index 10 (97% over 36bp)
TGATTTTGGTAGGATTGAGAAAATTGTTTATTCTGACCAGATCGGAAGAG264110.13989790011721104No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG260160.1378056025689812Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATGGTCTCTTGTTATGGACAAAGCGTGTAGATCGGAAGAGCACACGTCT257540.1364178001445857Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTTTATGAGTGGAACCTCTCGACCAAGAGATCGGAAGAGCACACGTCT256190.13570271110911475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTATGGAATTTAAGGGACATACGACGAGTAGATCGGAAGAGCACACGTCT256100.13565503850675004Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC251980.13347269270960901Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTGTGATGAAATCTTTCACAGACCTGTTCTGAGTGGCTGTGAGGAACA250210.1325351315297693No Hit
TACGATATGTCTTAATTCTCTGAATAGGCAGATCGGAAGAGCACACGTCT249030.1319100907432095Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGA240590.12743946003256143No Hit
TGATTCCACTTGTCAAGAACACAAATGATAAGATCGGAAGAGCACACGTC237430.1257656219939776No Hit
AAAGGAAAGGTTAACCGCTGAAAAAAAAAAAAAAAAGATCGGAAGAGCAC235860.12493399993050394No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT233610.12374218487138569No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC230700.12220077072825938Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC230100.12188295337916119No Hit
TGAAGACGAAGCTCAACCTGAGGAAAATCAGATCGGAAGAGCACACGTCT227900.12071762309913445Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTACGTGTTGAGATGCCTTTATCTGAAAAGATCGGAAGAGCACACGTCT225350.11936689936546706Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAATTGTTTACTCGCGATGGAAAAAAAAAAGATCGGAAGAGCACACGTC214200.11346079362805878No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAGATCGGAAGAGCACACGTC212740.11268743807858649No Hit
AGGGGTTAACCGTGAGGATACAAATGAAAAGATCGGAAGAGCACACGTCT212360.11248615375749095Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGTCAACCGTGAGGATACAAATGAAAAGATCGGAAGAGCACACGTCT211620.1120941790269365Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACTAGACGTGATACACTCAAGGAAGACCAGATCGGAAGAGCACACGTCT206600.10943510720614819Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGTGGTGATAGGCCAATATCATTTCTGAAAAGATCGGAAGAGCACAC206130.10918615028268795No Hit
GTATCAACGATTTAAGCCTAAAGACTCTTAGATCGGAAGAGCACACGTCT204960.10856640645194646Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCATGGAACTTCGAAGTACATATTCAGCAGATCGGAAGAGCACACGTC204230.10817972867721028No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG200750.10633638805244072Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTTTTATGTGCGGAACCTGTCAACCAAGTAGATCGGAAGAGCACACGTC198550.10517105777241395No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAGATCGGAAGAGCACA195730.10367731623165241No Hit
TTACATCTACCTTTGAAAAATTCGAAAAGTAGATCGGAAGAGCACACGTC194350.10294633632872653No Hit
ACTACATGGATAACCGTAGTAATTCTAGAGCTAATACATGCAAGGAGTCT192900.10217827773507254No Hit
GATACGATCCACTGAGATTCATCCTGTGTTTTTGGGTCTGTAGATCGGAA190080.10068453619431099No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)