FastQCFastQC Report
Fri 20 Dec 2024
1H12_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H12_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9741443
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC105710610.851636662042779No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2653662.724093340175578No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1423091.4608615992517742No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG1297651.332092175666377Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1158731.1894849664469627No Hit
CAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGA642680.6597379874829633Illumina DpnII expression Sequencing Primer (100% over 24bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT552760.5674313343515945Illumina RNA PCR Primer (100% over 50bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG524380.5382980734989672No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA506180.5196150098091218Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA500020.5132915113294817Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAGAATGAGTATCAAAACTACCATTATTAGAATCCTTTGATCGTCGGAC402100.4127725225102688No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT367770.3775313369898074No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA328290.33700346037029627Illumina RNA PCR Primer (100% over 45bp)
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT286930.2945456848641418No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT284640.2921949037734964Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCGGATCCTAACCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT250900.25755937801001355No Hit
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA239990.24635980521571596No Hit
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC234580.24080621320681136No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA230430.23654606406874218No Hit
TATCAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTC222790.22870328348685098Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAAGCTTACATAAGATTTCTTCATCCATGATCGTCGGACTGTAGAACTCT211040.2166414154453298Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT200310.2056266202040088Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGAA183630.18850390029485364Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA177630.1823446485289705Illumina DpnII expression Sequencing Primer (100% over 25bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT174420.17904944883422302Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA166310.17072419353067098Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG163540.16788067229875492No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC160270.1645238800863486No Hit
GGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG157530.16171115511326195Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT156910.16107469909745403No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT150120.15410447918239628Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT148820.15276997463312159No Hit
ACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGAACGT143640.1474524872752425Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT143270.1470726667496797No Hit
AATGAGTATCAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTA142460.14624116776128548No Hit
GGCGGGGATACTCACCACTATACTACCGAGGAGATCGTCGGACTGTAGAA142140.1459126743337717No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC127620.1310072850603345Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA119190.12235353632926867No Hit
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA117140.12024912530925859Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC110660.1135971334021048No Hit
AGCTTACATAAGATTTCTTCATCCATGATCGTCGGACTGTAGAACTCTGA109530.11243714098619681Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103640.10639080883602153No Hit
AGCCGGAGATCCTAACCACCTAGACTACGTTGGAGATCGTCGGACTGTAG103500.10624709296148424No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT101460.10415294736108398Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTAG99150.10178163543121897Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)