FastQCFastQC Report
Fri 20 Dec 2024
1H12_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H12_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9741443
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT102974210.570733719840069No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT2599122.6681057416237No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1369781.4061366473119024No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA1224371.2568671807657243Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1131001.161018957868973No Hit
AAAGGATTCTAATAATGGTAGTTTTGAGATCGGAAGAGCACACGTCTGAA589640.6052902018725562Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC512310.5259077120299324No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC471520.48403506544153674Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC464020.4763360007341828Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAGGATTCTAATAATGGTAGTTTTGATACTCATTCTGTAGATCGGAAGA395890.4063976969325797No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC357790.36728644821922174No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTGGGGGGGGGGG297370.30526278293677844TruSeq Adapter, Index 10 (96% over 32bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC273460.28071816464973415No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG267490.2745897091426804Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTGGGGGG256820.2636365064190182TruSeq Adapter, Index 10 (97% over 36bp)
TCCCATATGGTCTAGTGGTTAGGATCCGGCAGATCGGAAGAGCACACGTC241480.24788935273757695No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC235200.24144266922261928No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCAGATCGGAAGAGCACACGT225670.2316597243344749No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG223180.22910363485163338No Hit
AAAGGATTCTAATAATGGTAGTTTTGATAAGATCGGAAGAGCACACGTCT206950.21244285882491948Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGATGAAGAAATCTTATGTAAGCTTTAGATCGGAAGAGCACACGTCTG199550.2048464483136636Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG188050.19304121576238756Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAGGATTCTAATAATGGTAGTTTTAGATCGGAAGAGCACACGTCTGAAC174740.17937794226173678Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC166640.17106295237779454Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC160710.16497555854918003Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA160360.16461626886283687No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG158500.16270690081541306No Hit
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC154260.15835436290085567Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGTTTGGTCTAGTGGTATGATTCCAGATCGGAAGAGCACACGTCTGA153590.1576665797869987Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC152550.15659897614757895No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC142800.14659019202801885No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG141360.1451119716042069Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAGGATTCTAATAATGGTAGTTTTGATACTCATTAGATCGGAAGAGCAC139830.1435413624039067No Hit
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC137090.14072863743082006No Hit
AAAGGATTCTAATAATGGTAGTAGATCGGAAGAGCACACGTCTGAACTCC133480.13702282095168036Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCGGTAGTATAGTGGTGAGTATCCCCGCCAGATCGGAAGAGCACACG131520.13501079870815855No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT121260.12447847818849835Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC116020.11909939831296042No Hit
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT107480.11033272996618672Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT105960.10877238618549634No Hit
ATGGATGAAGAAATCTTATGTAAGCTAGATCGGAAGAGCACACGTCTGAA103530.10627788922031366Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCAACGTAGTCTAGGTGGTTAGGATCTCCGGCTAGATCGGAAGAGCACA101450.10414268194147418No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)