FastQCFastQC Report
Fri 20 Dec 2024
1H11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8802216
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1912972.173282273463864No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1101101.2509349918247858Illumina RNA PCR Primer (100% over 50bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC890071.0111885461570131No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT790770.898376045304955No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA685360.7786221106139636No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA649650.7380527812541751Illumina RNA PCR Primer (100% over 45bp)
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG647140.7352012266002106Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA473870.5383530692725559Illumina DpnII expression Sequencing Primer (100% over 25bp)
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC442400.5026007087306197No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT422760.48028814562151173Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT377440.42880111099295903No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC367370.41736081005056No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC318050.3613294652164864Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT303820.34516308166034554Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC301890.3429704519861817No Hit
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA254050.2886205019281508Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT228230.2592869795515129Illumina DpnII expression Sequencing Primer (100% over 22bp)
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC214640.24384768562825543No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT209830.23838315260611645Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA206100.23414558333946814No Hit
GAAGAACGAAAGTTTCAATCATCATACCAGGATCGTCGGACTGTAGAACT195090.2216373694987717No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT190210.21609331104803609No Hit
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA190080.21594562096635667Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC180000.20449395924844382No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC175590.1994838572468569No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG164680.18708925116129846Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA164060.1863848830794427No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG150820.1713432162991683No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACTC150030.17044571503357792Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTGAATCCATGTACGATTTTCGATGTGAGATCGTCGGACTGTAGAACTC148860.16911650429846303Illumina DpnII expression Sequencing Primer (100% over 21bp)
GATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGA144210.1638337436845449Illumina DpnII expression Sequencing Primer (100% over 24bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGGAT136570.15515411119199982No Hit
GGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTG134810.1531546147015706Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT131220.14907609629211555No Hit
TAAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACT129660.14730381531196235No Hit
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT128480.1459632438013337No Hit
AGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC127460.1448044446989258Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG126520.1437365318006284Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC126110.1432707400045625No Hit
AACTGAAATTAAGAGTGGATAACCCTCCCCAGGTCCGGACCTCGATCGTC125850.14297535984120363No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT125230.14227099175934788No Hit
AACTGAATTTAAGGGTGATAACCCTCCCCAAGTCCGGACCTCGATCGTCG118000.13405715106286872No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA115980.13176227440908062Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA115850.1316145843274012Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC111140.12626365906040024No Hit
GGTCAGAATAAACAATTTTCTCAATCCTACCAAAATCAGATCGTCGGACT108270.12300311648793893No Hit
CATCAAGTTTTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG104710.11895868040502529Illumina RNA PCR Primer (100% over 33bp)
AACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGGATCGTCGGACTG103920.1180611791394349No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG103480.11756130501682759No Hit
AGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAA101400.11519826370995666No Hit
AGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAACTC94990.10791600660560931Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA93780.10654135276843921No Hit
GGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG93050.10571201615593165Illumina DpnII expression Sequencing Primer (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)