FastQCFastQC Report
Fri 20 Dec 2024
1H11_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H11_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8802216
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1879872.1356781065131782No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT881511.0014637223171983No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC771130.876063482195847No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTGGGGGGGGGGG688560.7822575587783804TruSeq Adapter, Index 1 (96% over 33bp)
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC673180.7647846860381522No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA618790.7029934280185808Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTGGGGGG493750.5609382909939951TruSeq Adapter, Index 1 (97% over 37bp)
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC448040.5090081861204042Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG436250.49561383178963114No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC395760.4496140517342451Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC371130.4216324616437497No Hit
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA362790.41215757486523846No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT306250.3479237501101995Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT296650.3370174056169492No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG289640.3290535019817737Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT237780.27013652016719425Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG218480.2482102234255556Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT211480.24025768056589383No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC201830.2292945321950745No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG199860.22705645941885544Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGTATGATGATTGAAACTTTCGTTCTTCAGATCGGAAGAGCACACGTC190170.21604786794598088No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC184280.20935637116835126No Hit
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC180650.20523240965684095Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCAGATCGGAAGAGCACACGT174690.19846138745061473No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT169900.19301957597950337No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAGATCGGAAGAGCACAC161160.18309025818044No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA157660.17911398675060916Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA149180.1694800491149047No Hit
TACCACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCT146220.16611725956281917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACATCGAAAATCGTACATGGATTCACTAGATCGGAAGAGCACACGTCT145180.1649357389093837Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTTATCAGATCGGAAGAGCACACGTCTGAA137020.15566534609012095Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGA135560.15400667286510578No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCAGATCGGAAGAGCACACGTCTGA128370.14583827527068183Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC127620.14498621710714665No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC125780.142895834412607No Hit
TAGTAACAATGACCTGATCTGGAGACTCTAGATCGGAAGAGCACACGTCT125210.14224827020832026Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCACCCCTCCCCGCACATTGACCTCTTAAGATCGGAAGAGCACACGTC124230.14113491420796762No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT123090.13983978579939416No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACTCTTAATTTCAGTTAGATCGG123000.1397375388197699No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA121010.13747674449252326Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC119230.13545452645106643No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACCCTTAAATTCAGTTAGATCGGA115560.1312851218375009No Hit
GTTCGCGCTAGTCCCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT109590.12450273885576087Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC109300.12417327636586059Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTAGGGGG108690.12348026905951864TruSeq Adapter, Index 1 (97% over 37bp)
TGATTTTGGTAGGATTGAGAAAATTGTTTATTCTGACCAGATCGGAAGAG106810.12134444326292379No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT106440.1209240945689131No Hit
CCTTGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTAGATCGGAAGAGC102870.11686829771048564No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC101340.11513009905687387No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTAGATCGGAAGAGCACACG99350.11286930472962718No Hit
TAAAACTTGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCA96590.10973373068781771TruSeq Adapter, Index 1 (97% over 37bp)
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTCAGATCGGAAGAGCAC93520.10624597260508037No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTAGATCGGAAGAGCACACGTCT91720.10420103301259592Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGTTTGGTCTAGTGGTATGATTCCAGATCGGAAGAGCACACGTCTGA91470.10391701362475085Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTAGGGGGGGGGG90940.10331489252251934TruSeq Adapter, Index 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)