FastQCFastQC Report
Fri 20 Dec 2024
1G5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17744776
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA5183262.9210061597847163Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG3256071.834945676406397Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2422541.3652130632700012No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1973351.1120737731487849Illumina RNA PCR Primer (100% over 50bp)
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1854551.0451244918504465Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1245570.7019361641984097Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1129210.6363619354789263No Hit
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG1049380.5913740472125429No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC982300.5535713722168146No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT759750.4281541790102056No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC744540.41958264223791836No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC715980.40348776451165125Illumina DpnII expression Sequencing Primer (100% over 21bp)
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT658300.37098242322134695No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT591120.33312339361173116Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT542680.30582521864463097Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTGTTCTCGCTGTTATTATATTCAACCAAGATCGTCGGACTGTAGAACT538020.3031990936374739No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC508410.2865124924653881No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA494690.2787806394400245No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA484490.2730324688235005Illumina RNA PCR Primer (100% over 45bp)
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT463900.2614290538240663No Hit
TTTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAA386490.21780494721376026No Hit
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT374250.2109071424739315Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG373850.2106817240183815Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGATCGTCGGAC371370.20928412959397177No Hit
TTTCATTTGTATCCTCACGGTTGACCCCTGATCGTCGGACTGTAGAACTC370490.20878820899176187Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT346840.19546034280737046No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG345990.1949813285893268No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGGATCGTCGGACT309320.17431609167678422No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT302090.1702416530927187No Hit
GCTCCAGATTGAGACAACAGATACGTAAGATCGTCGGACTGTAGAACTCT289500.16314660720428367Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC287290.16190117023737013No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT284020.1600583743632492Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC276990.15609664500695866No Hit
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC276870.15602901947029368Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTGAATCCATGTACGATTTTCGATGTGAGATCGTCGGACTGTAGAACTC270170.15225326033983186Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG268120.1510979907551383Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT256470.14453267823724572No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG231140.13025805453954448No Hit
GCTTTCAGAGTTAGAAAGAACAGAATATGAGATCGTCGGACTGTAGAACT225910.12731070823322876No Hit
AGGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAAC220400.12420556900802807No Hit
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATTCAGAGACTCTAGTCT219980.12396887962970059No Hit
TTTTTTTTCAAGAATCCATCCCGCCTATCAATCACTGATCGTCGGACTGT211130.11898149630065773No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTAGATCGTCGGACTG210180.11844612746872657No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA207550.1169640011234856Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCTATTTTCAGTAAGGACCAACTAAGCCAGATCGTCGGACTGTAGAACTC204990.11552132300796585Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT204920.11548187477824459Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTCGTCGTATGTCCCTTAAATTCCATAAGATCGTCGGACTGTAGAACTC196840.1109284219761354Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTTTTTCAAGAATCCATCCCGCCTATCAATCACTGATCGTCGGACTGTA194890.10982950700532934No Hit
TTTTTTTTTCCATCGCGAGTAAACAATTAAGATCGTCGGACTGTAGAACT186410.10505063574767019No Hit
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC184670.10407006546602786No Hit
AGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC181690.10239069797218064Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)