FastQCFastQC Report
Fri 20 Dec 2024
1G5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17744776
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC4894042.7580173454993178Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA3102321.748300457554381Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2380731.3416511992036417No Hit
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC1753610.9882401445924142Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC1173540.6613439358152506Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1096300.6178156320485534No Hit
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA1059190.5969024348349058No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT946500.5333964204450933No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT735570.41452763337221055No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC731210.41207057220671595No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT682770.38477239723961576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA646230.3641804213251269No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC546600.3080343195090206Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGTTGAATATAATAACAGCGAGAACAGTAGATCGGAAGAGCACACGTC517080.2913984374894335No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG516890.2912913637230473Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT493760.2782565415308708No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG483010.27219842053796567No Hit
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC448700.2528631525131678No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAAGATCGGAAGAGCACACG377850.21293590857388114No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGA367160.20691160034930844No Hit
GGCTGTTTGGTCTAGTGGTATGATTCCAGATCGGAAGAGCACACGTCTGA365570.20601556198849733Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGTCAACCGTGAGGATACAAATGAAAAGATCGGAAGAGCACACGTCT356020.20063369636224207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG353460.1991910182467223Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC340320.1917860219819061No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC339300.1912112049202537No Hit
TATGATTGAAATCTTCATGAACGACACAAAAGGCCAAAAAAAAAAAAAAA312770.17626032585590262No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAG303580.17108133683964227No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT284700.16044158573768416No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC284350.16024434458907794No Hit
TTACGTATCTGTTGTCTCAATCTGGAGCAGATCGGAAGAGCACACGTCTG275490.1552513257986463Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG273720.15425384913283774No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG265720.14974548002183855Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT262400.14787450684077388Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCACATCGAAAATCGTACATGGATTCACTAGATCGGAAGAGCACACGTCT261140.14716443870579152Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA256730.1446792002333532Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC247410.13942695021903911No Hit
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAA239870.13517781233192236No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGCAGATCGGAAGAGCACACGTC215110.12122440993337982No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT215100.12121877447199107No Hit
AGTGATTGATAGGCGGGATGGATTCTTGAAAAAAAAAGATCGGAAGAGCA208850.11769661110402295No Hit
TAGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGC207110.11671604082238063No Hit
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA201400.11349819236940493Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG195820.110353604914483Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGCTTAGTTGGTCCTTACTGAAAATAGCAGATCGGAAGAGCACACGTCT194140.10940684740117317Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGATTGATAGGCGGGATGGATTCTTGAAAAAAAAGATCGGAAGAGCAC190280.10723155930511606No Hit
TTATGGAATTTAAGGGACATACGACGAGTAGATCGGAAGAGCACACGTCT188560.10626225994625123Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAATTGTTTACTCGCGATGGAAAAAAAAAAGATCGGAAGAGCACACGTC187450.10563672373210009No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT182140.10264429373467436No Hit
GAGACTCTGCACGGTGGATCTCTCGGCTCGCGCATCGATGAAGAACGCAG179470.10113962554387837No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)