FastQCFastQC Report
Fri 20 Dec 2024
1F8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10224118
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC107334910.498206300044659No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT5172725.059331279236018Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG2185052.137152564162503Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1668991.6324048685666577No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1040031.0172319998654162No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA956400.9354352130912418Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT845730.8271911572225595No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT832150.8139088379066047Illumina RNA PCR Primer (100% over 50bp)
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG784590.7673913779164129Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG637320.623349613140224No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA465350.4551492852488596Illumina RNA PCR Primer (100% over 45bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA439540.42990505391271894No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC426360.4170139663881032No Hit
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC378520.3702226441439741No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT356330.3485190605194502No Hit
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT326480.3193233880907869Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA294090.2876433937871218No Hit
GGCGGGGATACTCACCACTATACTACCGAGGAGATCGTCGGACTGTAGAA280520.27437085526595056No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG264550.2587509259967461No Hit
AACGCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAG223010.21812150446620435No Hit
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA219900.2150796772885446Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG208670.20409584474670575Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCGGATCCTAACCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT189510.18535584194157384No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA188460.1843288584893093Illumina DpnII expression Sequencing Primer (100% over 25bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT180750.17678786571125255Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA176580.1727092742865448No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC173160.16936424247059745No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT165100.1614809218751192No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT163150.1595736668923422No Hit
GCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTAG161140.15760772714086438Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA156030.15260974100651029No Hit
CCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACTC149130.14586099260591476Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT143980.1408238832924268No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA139920.13685288061033724No Hit
GGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG137690.13467176337362302Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC132220.12932166862706398Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC131030.12815775404783084No Hit
AGCCGAGATATCCGTCGCTGAAAGTTGTGGATCGTCGGACTGTAGAACTC125580.1228272208908387Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTC117280.11470916122055712Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT114250.11174558040116515Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGCAACAGCTCGCAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA113670.11117829430372381Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCATGCACTACCAATGGATAGAATTCTACCAAATTAAAGGTATTGATC110190.10777457771907561No Hit
GGCAGGATCAACCAGGTAGATCGTCGGACTGTAGAACTCTGAACGTGTAG110170.10775501612950866Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTC110040.10762786579732356Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)