FastQCFastQC Report
Fri 20 Dec 2024
1F8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10224118
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT104257210.197182778993747No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG4952394.843831027771784Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA2078062.032507840774138Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1614181.5787963323584489No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1009860.9877233420036818No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC907920.8880179199809706Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC822230.8042062894814007No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA748790.732376132591584Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC610490.5971077407361691No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG423080.41380586569912436No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT406570.39765777351161247No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCAGATCGGAAGAGCACACGT362610.3546613996434705No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC341070.33359356767987225No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG310180.30338069259372785Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC283170.2769627658835706No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCCCGCCAGATCGGAAGAGCACACG256850.2512197140134728No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA256360.2507404550690827No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGGGGG230580.2255255661172925TruSeq Adapter, Index 14 (97% over 43bp)
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCGTTAGATCGGAAGAGCACA213510.20882974942190613No Hit
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT205420.20091708644207742Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA198930.19456935062760428Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCATATGGTCTAGTGGTTAGGATCCGGCAGATCGGAAGAGCACACGTC183860.17982969288891226No Hit
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC178000.1740981471457978Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC170680.16693860536429644Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG170230.16649846959904022No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC169790.16606811462856746No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC159910.15640468938249733No Hit
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA156990.1535486973057236No Hit
GTTCGCGCTAGTCCCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC152750.14940164031753156Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC151230.14791495951044384No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGAGATCGGAAGAGCACACGTCT141910.13879925877224814Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC137310.13430009317185113No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG135910.13293078190216503No Hit
GGCTGTTTGGTCTAGTGGTATGATTCCAGATCGGAAGAGCACACGTCTGA135510.1325395501108262Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGGGGG128150.12534088515019096TruSeq Adapter, Index 14 (97% over 43bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT127440.12464644872056446Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT125290.12254357784211801No Hit
CACAACTTTCAGCGACGGATATCTCGGCTAGATCGGAAGAGCACACGTCT121470.1188073142348318Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGTTTGGTCTAGTGGTATGATTCTCAAGATCGGAAGAGCACACGTCT111260.10882112276090711Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG109540.10713882605814995Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATACCTTTAATTTGGTAGAATTCTATCCATTGGTAGTGCATGGCTAGAT109060.10666934790854331No Hit
CTTGCGAGCTGTTGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA107810.10544674856060934Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACCTGGTTGATCCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC105790.10347102801434803Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT104460.10217018230814628Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)