FastQCFastQC Report
Fri 20 Dec 2024
1F4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17139209
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3063501.7874220449730207No Hit
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2117431.2354304098864772Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1731461.010233319402313Illumina DpnII expression Sequencing Primer (100% over 25bp)
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG1507010.8792762839872015No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1403340.8187892451746168No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1062800.6200986288223687Illumina RNA PCR Primer (100% over 50bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT987180.5759775728273108No Hit
CAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGA920280.536944266214386Illumina DpnII expression Sequencing Primer (100% over 24bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG791930.46205749635236965No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC780280.4552602165012399No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC765490.446630880106544No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGATCGTCGGAC737400.4302415589890992No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA731400.42674081400139297Illumina RNA PCR Primer (100% over 45bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT668650.390128856004965Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT661290.3858346088200453Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGGATCGTCGGA600170.35017368654527753No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG591680.3452201323876732Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA539160.3145769445952844Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC505200.294762727964867No Hit
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA502470.29316988899546065Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG491910.2870085778170976No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGGATCGTCGGACT486420.28380539615334643No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC469930.2741841820121337No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG430370.2511026033931904No Hit
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC406710.23729799899166876No Hit
GGCGAGAATTCTACCATTGAACCACCAGTGCGATCGTCGGACTGTAGAAC392390.2289428876210098No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT369910.21582676306707038Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC358320.20906449066581778Illumina DpnII expression Sequencing Primer (100% over 21bp)
TATCAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTC346810.20234889486440127Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG343200.20024261329679802Illumina DpnII expression Sequencing Primer (100% over 23bp)
GATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGA342610.19989837337300692Illumina DpnII expression Sequencing Primer (100% over 24bp)
TAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG336600.19639179381032112No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA330640.1929143871225329No Hit
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATTCAGAGACTCTAGTCT327630.19115818005370028No Hit
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA316340.18457094490183298No Hit
AACTGAATTTAAGGGTGATAACCCTCCCCAAGTCCGGACCTCGATCGTCG285690.1666879725896335No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACGATCGTC284360.1659119741173586No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT282520.1648384123211287No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT273590.1596281368644259No Hit
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT269600.1573001414476012No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT254340.1483965800288683No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC253720.14803483638013867No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT246190.1436414014205673Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACTC245340.14314546254730892Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC245120.14301710189775968Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTCGTCGTATGTCCCTTAAATTCCATAAGATCGTCGGACTGTAGAACTC240320.14021650590759469Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT229450.13387432290486684No Hit
GCTTTCAGAGTTAGAAAGAACAGAATATGAGATCGTCGGACTGTAGAACT229130.13368761650552252No Hit
ACTGCTCGTCAGTAGTAACTCAGGCCTTGGAGATCGTCGGACTGTAGAAC227430.13269573875900575No Hit
TATCAGACTAACATGAGATATCAGCATCAAAGCTAAGACAGGGGTAACTG216100.12608516530722041No Hit
GGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTG212940.12424143961369512Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT212300.12386802681500644Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAA209900.12246772881992396No Hit
ACTTGGTTGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT206440.12044896587701334No Hit
GATTTTCCTCAGGTTGAGCTTCGTCTTCAGATCGTCGGACTGTAGAACTC201940.11782340713623364Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCT201010.11728079166313918Illumina DpnII expression Sequencing Primer (100% over 22bp)
TAAGGGTGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA198720.11594467399283129No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC195770.11422347437387573No Hit
CATCAAGTTTTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG193050.11263646997944887Illumina RNA PCR Primer (100% over 33bp)
AGCATCAAAGCTAAGACAGGTAACTAAGTGTCATCACCACTGATCGTCGG188320.10987671601414044No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT187930.10964916758993953No Hit
AGTGGATAACCCTCCCCAGGTCCGGACCTCGATCGTCGGACTGTAGAACT187540.10942161916573862No Hit
GGCGAGAATTCTACCATTGAACCACAGTGCGATCGTCGGACTGTAGAACT186420.10876814676803347No Hit
TAAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACT183190.10688357904965158No Hit
ACTTGGTCGACAGGTTCTGCACATAAAAGAGATCGTCGGACTGTAGAACT171520.10007463004856293No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)