FastQCFastQC Report
Fri 20 Dec 2024
1F4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17139209
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC72

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3011201.7569072178301812No Hit
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC2004921.1697856067920054Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC1644180.959309148981146Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA1476730.8616091909492439No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1361090.7941381658861854No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC949600.5540512400543106No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTGGGGGGGGGGG879300.5130341779483522TruSeq Adapter, Index 7 (96% over 31bp)
AAAGGATTCTAATAATGGTAGTTTTGAGATCGGAAGAGCACACGTCTGAA865810.5051633363009926Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC774230.4517302986386361No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG767820.4479903360767699No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT741080.43238868258155905No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGA728330.4249495994826833No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTGGGGGG664430.38766666536361155TruSeq Adapter, Index 7 (97% over 35bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG638080.3722925602926016Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC617290.3601624789101994Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAG591750.34526097441252973No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA565990.3302311092653109Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA524630.3060993071500558Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT497810.2904509770550088No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA487490.284429695676154No Hit
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC477790.2787701579460289Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAG475940.2776907615748195No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT454410.2651289216439335No Hit
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA426750.24899048725060768No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT399450.23306209755654417No Hit
GCACTGGTGGTTCAATGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT384870.22455528723641796No Hit
TTTTATGATGAATCTTTTAGACTAGAGTCTCTGAATGACAAAGTCTTGAA384060.22408268666307762No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG349710.2040409216084593Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT340730.1988014732768589Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA329490.1922434109998892Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGGATTCTAATAATGGTAGTTTTGATAAGATCGGAAGAGCACACGTCT328190.19148491625255284Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTTATCAGATCGGAAGAGCACACGTCTGAA327150.19087812045468375Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAGATCGGAAGAGCACA327110.19085478215476573No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC324660.18942531128478565No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAGATCGGAAGAGCACAC308690.1801074950425075No Hit
GTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGG280840.16385820372457094No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACCCTTAAATTCAGTTAGATCGGA280080.16341477602612814No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC268140.15644829350059272No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGTAGATCGGAAGAGCACACGTC262740.15329762301165706No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC260540.15201401651616478No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC244650.1427428768737227No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT243750.14221776512556678No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG237710.13869368183794245Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCT237410.13851864458855717Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT233500.13623732577156855Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTATGGAATTTAAGGGACATACGACGAGTAGATCGGAAGAGCACACGTCT230170.1342944123033916Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC224530.1310037120149477No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGTAGATCGGAAGAGCACACGT223100.13016936779287772No Hit
AGTGGTGATGATATCCCAGTTACCCCTGTCTTAGCTTTGATGCTGATATC220780.1288157463976313No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGCAGATCGGAAGAGCACACGTC218240.12733376435283564No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTAGATCGGAAGAGCACACG205020.11962045622992286No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCAGATCGGAAGAGCACACGTCTGA204550.11934623120588587Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG203070.11848271410891834Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTTTATGTGCGGAACCTGTCAACCAAGTAGATCGGAAGAGCACACGTC198150.1156121032189992No Hit
TGAAGACGAAGCTCAACCTGAGGAAAATCAGATCGGAAGAGCACACGTCT194050.11321992747739991Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT193300.11278233435393664No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACCCTTAAGATCGGAAGAGCACAC191610.11179629118239938No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCAAGATCGGAAGAGCACACGTCTG190250.11100278898518595Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTAGATCGGAA185850.10843557599420135No Hit
GCACTGTGGTTCAATGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC181580.10594421247795041No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC181530.10591503960305285No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC179460.1047072825822942No Hit
TAAAACTTGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACC178960.10441555383331869TruSeq Adapter, Index 7 (97% over 35bp)
TACCACCCCTCCCCGCACATTGACCTCTTAAGATCGGAAGAGCACACGTC174350.10172581476776436No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)