FastQCFastQC Report
Fri 20 Dec 2024
1F11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10176436
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA6714036.597624158398874Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2487102.443979404970463Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2224462.1858929786420314Illumina RNA PCR Primer (100% over 45bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1710571.680912649575942No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1480331.4546644817497991Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1309951.287238479168935Illumina RNA PCR Primer (100% over 50bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG1041121.023069373206887No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG793540.779781841108223Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT711360.6990266533391455No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC655810.6444397626045111No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG628170.6172789766476201Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT610390.5998072409633393Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACTCT424500.4171401461179533Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT369680.3632705988619198Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG339070.3331913058756523Illumina RNA PCR Primer (100% over 33bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT337120.3312751143917183Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTGA330650.324917289314255Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC326750.32108490634638687Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC292670.2875957751810162Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCTACTTCAGATGTAGTATCGCCAAATCGTAGATCGTCGGACTGTAGAAC285430.28048130013297384No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT283750.27883042746989223No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC267190.2625575397909445No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT249240.24491875151575662No Hit
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT223840.2199591291096411No Hit
TTTCATTTGTATCCTCACGGTTGACCCCTGATCGTCGGACTGTAGAACTC217760.2139845423289647Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAA211710.20803943541727182No Hit
TCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC208510.20489491605902105Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT206690.20310647067401594Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTTTCAGAGTTAGAAAGAACAGAATATGAGATCGTCGGACTGTAGAACT204950.20139663827296708No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT193730.19037116727310033Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGGATCGTCGGACT173460.1704526024631806No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA165470.16260113069054824No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA155200.15250918887516218Illumina DpnII expression Sequencing Primer (100% over 24bp)
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG154440.15176236552757763Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCTCCAGATTGAGACAACAGATACGTAAGATCGTCGGACTGTAGAACTCT144300.1417981698111205Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG142920.14044209583787487Illumina RNA PCR Primer (100% over 33bp)
ACTGTTCTTGCTGTTACTATATTCAACCAAGATCGTCGGACTGTAGAACT136290.13392704479249906No Hit
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT135330.13298368898502383Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAACT130950.1286796281134181No Hit
AGCCGAGATATCCGTCGCTGAAAGTTGTGGATCGTCGGACTGTAGAACTC125380.12320619910546285Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG122300.12017959922314649No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT114050.11207263525265622No Hit
CTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAACG112300.11035297622861286Illumina Small RNA Adapter 1 (100% over 26bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC109840.10793562697195758No Hit
ACTGTTCTCGCTGTTATTACATTCAACCAAGATCGTCGGACTGTAGAACT108660.1067760854586026No Hit
GCTGAATATGTACTTCGAAGTTCCATGAGAGATCGTCGGACTGTAGAACT107160.10530209200942255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)