FastQCFastQC Report
Fri 20 Dec 2024
1F11_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F11_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10176436
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC6391226.280410941512334Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC2369232.328152999733895Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTGGGGGG1786951.75596839600819TruSeq Adapter, Index 3 (97% over 37bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1684141.6549408850013894No Hit
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC1403021.3786948593790598Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC1017891.0002421279905853No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA756950.7438262275712243Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC692610.6806017352243948No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT635950.6249240893373672No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA598400.5880251199928934Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG589600.5793776917577037Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTGGGGGGGGGGG448570.4407928276657958TruSeq Adapter, Index 3 (96% over 33bp)
TCCCATATGGTCTAGTGGCTAGGATCCGAGATCGGAAGAGCACACGTCTG403980.3969759157331702Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG355380.34921852797973674Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAG319010.31347910014861785TruSeq Adapter, Index 3 (97% over 37bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC317010.3115137755497111Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAA314310.30886058734118704Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT310350.30496924463535174Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT282210.27731712752873405Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACGATTTGGCGATACTACATCTGAAGTAGCAGATCGGAAGAGCACACGT279560.2747130724351826No Hit
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA278560.27373041013572924No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT258480.2539985511627057No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC239060.23491524930732136No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC218440.21465275269259296No Hit
AGGGGTCAACCGTGAGGATACAAATGAAAAGATCGGAAGAGCACACGTCT209840.20620185691729404Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAAGATCGGAAGAGCACACG207620.20402034661250756No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGCAGATCGGAAGAGCACACGTC198060.19462609502973338No Hit
TGAAAATCTTTCTTCTGAGGCGGAAGATCGGAAGAGCACACGTCTGAACT196680.19327002105648775Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG194180.19081336530785434Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG186610.18337461170099237Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAG170260.16730808310492987No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG161070.15827741657295344No Hit
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA150070.1474681312789664Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA148620.14604327094475905Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACGTATCTGTTGTCTCAATCTGGAGCAGATCGGAAGAGCACACGTCTG139010.13659988624701222Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTGGGGGGGGGGT135080.13273802341016047TruSeq Adapter, Index 3 (96% over 33bp)
GAGGTCCGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAG134840.13250218445829168TruSeq Adapter, Index 3 (97% over 37bp)
TTGGTTGAATATAGTAACAGCAAGAACAGTAGATCGGAAGAGCACACGTC130860.12859118850646728No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG129520.12727442102519979Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAGATCGGAAGAGCACACGTC124930.12276400107070884No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA121000.11890213823385712No Hit
CACAACTTTCAGCGACGGATATCTCGGCTAGATCGGAAGAGCACACGTCT120920.11882352524990084Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC109660.10775874775805595No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTGGGGTG108070.10619631470192512TruSeq Adapter, Index 3 (97% over 37bp)
TTAACGAGTAGATAAATGAAGAGAGATCGGAAGAGCACACGTCTGAACTC106550.104702668006756Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT106530.10468301476076693No Hit
TTGGTTGAATGTAATAACAGCGAGAACAGTAGATCGGAAGAGCACACGTC104320.10251133107897499No Hit
TCTCATGGAACTTCGAAGTACATATTCAGCAGATCGGAAGAGCACACGTC103660.10186277396133578No Hit
AAAGGAAAGGTTAACCGCTGAAAAAAAAAAAAAAAAGATCGGAAGAGCAC103570.10177433435438499No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)