FastQCFastQC Report
Fri 20 Dec 2024
1E9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12088004
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2211941.8298637227452934No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1115650.9229398004831899Illumina RNA PCR Primer (100% over 50bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1034090.8554679498782429No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC987410.8168511525972361No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA908000.7511579248319242No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA577410.4776719134110148Illumina RNA PCR Primer (100% over 45bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT472650.3910074814667501Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT463780.38366962817020905No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC459570.3801868364702725No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG448990.3714343575663939Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA369040.3052944059250807Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG353310.2922815048704484No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC344800.28524146749124174No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC306680.25370607091129355No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT248140.20527789368699748Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC247570.2048063518178849No Hit
GGCGAGAATTCTACCATTGAACCACCAGTGCGATCGTCGGACTGTAGAAC230750.19089173034687942No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA226450.1873344846676093Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT210240.17392449572319796Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA201680.16684309502213931No Hit
GGTGCTCTAACCAATTGAACTACAGGCCCGATCGTCGGACTGTAGAACTC197160.16310385072672048Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT194280.16072132338804654No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT189470.1567421718258862No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG182470.15095130676660928No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT181150.14985931506971706Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG178590.14774151299089577No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT171170.1416031960280622No Hit
AGAACCGGTGTTTTCCCTGTGGTATGGTCGTAGACGATCGTCGGACTGTA161390.13351253027381527No Hit
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT160540.13280935380233164No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC159920.1322964486113671No Hit
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC144470.11951518215910584No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT142780.11811710188050899No Hit
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC141910.11739738008028455No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCAGATCGTCGGACTG141520.11707474616983914No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG139870.11570975654872384No Hit
GCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTAG136450.11288050533404853Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGAGGTCAATGTGCGGGGAGGGGTGGTAGATCGTCGGACTGTAGAACTC122880.10165449978342164Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)