FastQCFastQC Report
Fri 20 Dec 2024
1E9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12088004
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2138081.7687618237055514No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC993630.8219967498356221No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT958190.7926784273069399No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC875340.7241394029982121No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC448930.37138472158017155No Hit
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA444140.36742211534675207No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC430870.356444289727237Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTGGGGGG422120.34920570840314086TruSeq Adapter, Index 21 (97% over 43bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA421970.3490816184375849Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT338380.27993041696544774Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTGGGGGGGGGGG337730.27939269378137205TruSeq Adapter, Index 21 (97% over 39bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC335920.2778953415303304No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT335670.27768852492107055No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG301220.24918919616505752No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT236940.19601250959215433No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG232970.19272826183710726Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGGTGGTTCAATGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT221080.18289206390070686No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC209870.17361840714149335Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG196770.16278121681627505Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC194560.16095295799041762No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC186320.1541362825492116No Hit
GGGCCTGTAGTTCAATTGGTTAGAGCACCAGATCGGAAGAGCACACGTCT181220.14991722372030983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC179780.14872596005097286No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA177870.1471458811562273No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG169930.14057738564613315Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC158050.13074946037410312No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC156500.1294671973966918No Hit
GTCTACGACCATACCACAGGGAAAACACCGGTTCTAGATCGGAAGAGCAC154660.127945027152539No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT152590.1262325856278671No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT139720.1155856665831679No Hit
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA138580.11464258284494279No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGCAGATCGGAAGAGCACACGT137420.11368295377797691No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGTAGATCGGAAGAGCACACGTC136530.11294668664901171No Hit
TGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGC135790.11233450948560243No Hit
GTTCGCGCTAGTCCCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC127150.10518692746958058Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)