FastQCFastQC Report
Fri 20 Dec 2024
1E5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14531670
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3904482.6868763191016587No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG1375680.9466771541054813Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1004750.6914208759213497No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT982060.6758067035653851Illumina RNA PCR Primer (100% over 50bp)
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA967830.666014298425439Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC715640.49246920691152496No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA604160.41575400487349357Illumina RNA PCR Primer (100% over 45bp)
CACGCACGCTACCAAACTGCGCTACATTCCGGATCGTCGGACTGTAGAAC467750.32188316965634367No Hit
AGTCTATAATCCGAACTAAGGTTAAGTTTTTGAGGGATCGTCGGACTGTA452610.31146454605699136No Hit
GATTATGATACCACTACACCATGACCGCGATCGTCGGACTGTAGAACTCT391350.26930834515234653Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGGAGATCCTAACCACCTAGACTACGATGGAGATCGTCGGACTGTAGAAC377940.26008022477801934No Hit
CTTGCGAGCTACCAGCCTGCTCTATCCCGCAGATCGTCGGACTGTAGAAC365250.25134757395399154No Hit
AGTTCAGCGGGTAGCCTCGTCGGCCGTCAGGTCAAAGATCGTCGGACTGT334930.23048280066915913No Hit
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT295840.2035829330008182Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAGATCGT292280.2011331113354487No Hit
CCTTGCGAGCTACCAGCCTGCTCTATCCCGCAGATCGTCGGACTGTAGAA290330.19979121463672106No Hit
AGTCGTTAGCCGACGGATGGGAGCTCCGCAGATCGTCGGACTGTAGAACT290010.19957100594769905No Hit
GGAGCTATTCTACCACTAGACCACCGACGCGATCGTCGGACTGTAGAACT260660.17937374025146458No Hit
TTCGCCTTCCACAAATCCAAGAATTTCGATCGTCGGACTGTAGAACTCTG260340.17915353156244257Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACACGCACGCTACCAAACTGCGCTACATTCCGGATCGTCGGACTGTAGAA259530.1785961283183557No Hit
ACAGACCCAAAAACACAGGATGAATCTCAGTGGATCGTATCACAAAGGGA257860.17744691422252226No Hit
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG253470.174425926270002No Hit
AGCCATGCACTACCAATGGATAGAATTCTACCAAATTAAAGGTATTGATC244620.16833577971423794No Hit
CAGTCGTTAGCCGACGGATGGGAGCTCCGCACCCTCGGCTTGCGATCGTC243280.16741365582895842No Hit
GGGAGCTATTCTACCACTAGACCACCGACGCGATCGTCGGACTGTAGAAC236450.16271357662264557No Hit
CAGTCTATAATCCGAACTAAGGTTAAGTTTTTGAGGGATCGTCGGACTGT223410.153740072545No Hit
CGGAGATCCTAACCAACTAGACTACGATGGAGATCGTCGGACTGTAGAAC217220.1494804107167311No Hit
GACGGAGACTCCTTGCATGTATTAGCTCTAGAATTACTACGGTTATCCAT202150.13910995776810234No Hit
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA200550.13800891432299248Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCCGGAGATCCTAACCACCTAGACTACGTTGGAGATCGTCGGACTGTAG199520.13730011760520297No Hit
CGGAGATCCTAACCACCTAGACTACGTTGGAGATCGTCGGACTGTAGAAC199320.13716248717456422No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC190580.13114803735565148No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT186940.1286431635180265No Hit
TCTCCATCCTCTAAACGCCAGGGTTCAAGATCGTCGGACTGTAGAACTCT186280.12818898309691865Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCACGCTACCAAACTGCGCTACATTCCGGATCGTCGGACTGTAGAACTC152460.1049156772759084Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT151490.10424816968731054Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAAACGTAAAGAATACGGGCCGAACCTCGATCGTCGGACTGTAGAACTC146100.1005390295815966Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)