FastQCFastQC Report
Fri 20 Dec 2024
1E5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14531670
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3859522.6559369982940706No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA1309140.9008875098319739Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC978650.6734601047229947No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC914980.6296454571291531Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT706930.48647540165720804No Hit
CGGAATGTAGCGCAGTTTGGTAGCGTGCGTGAGATCGGAAGAGCACACGT448380.30855366244898214No Hit
CCTCAAAAACTTAACCTTAGTTCGGATTATAGACTAGATCGGAAGAGCAC444700.3060212625252294No Hit
GCGGTCATGGTGTAGTGGTATCATAATCAGATCGGAAGAGCACACGTCTG371010.2553113303563871Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCATCGTAGTCTAGGTGGTTAGGATCTCCGAGATCGGAAGAGCACACGT362710.24959966748487958No Hit
TGCGGGATAGAGCAGGCTGGTAGCTCGCAAGAGATCGGAAGAGCACACGT353570.24330995680468936No Hit
TTTGACCTGACGGCCGACGAGGCTACCCGCTGAACTAGATCGGAAGAGCA329830.2269732246878714No Hit
TGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCG290650.200011423325743No Hit
TGCGGGATAGAGCAGGCTGGTAGCTCGCAAGGAGATCGGAAGAGCACACG281090.19343268874121144No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG280560.1930679681000188Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGGAGCTCCCATCCGTCGGCTAACGACTAGATCGGAAGAGCACACGTC280160.1927927072387413No Hit
CCTTTGTGATACGATCCACTGAGATTCATCCTGTGTTTTTGGGTCTGTAG256830.17673811750473278No Hit
GCGTCGGTGGTCTAGTGGTAGAATAGCTCCAGATCGGAAGAGCACACGTC253580.17450162300685332No Hit
CGGAATGTAGCGCAGTTTGGTAGCGTGCGTGTAGATCGGAAGAGCACACG252720.17390981215510676No Hit
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA250700.17251974480565552No Hit
GAAATTCTTGGATTTGTGGAAGGCGAAAGATCGGAAGAGCACACGTCTGA244800.1684596471018128Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATACCTTTAATTTGGTAGAATTCTATCCATTGGTAGTGCATGGCTAGAT241810.1664020721637637No Hit
GCAAGCCGAGGGTGCGGAGCTCCCATCCGTCGGCTAACGACTGAGATCGG241600.16625756021159302No Hit
GCGTCGGTGGTCTAGTGGTAGAATAGCTCCCAGATCGGAAGAGCACACGT233780.16087621037361846No Hit
CCTCAAAAACTTAACCTTAGTTCGGATTATAGACTGAGATCGGAAGAGCA218290.15021673352064835No Hit
TCCATCGTAGTCTAGTTGGTTAGGATCTCCGAGATCGGAAGAGCACACGT208430.14343155329015866No Hit
TCCAACGTAGTCTAGGTGGTTAGGATCTCCGGCTAGATCGGAAGAGCACA196070.1349259926766848No Hit
TCCAACGTAGTCTAGGTGGTTAGGATCTCCGAGATCGGAAGAGCACACGT191370.1316916775566745No Hit
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC189060.1301020460827971Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT185510.12765910593895954No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC183280.12612452663733764No Hit
TTGAACCCTGGCGTTTAGAGGATGGAGAAGATCGGAAGAGCACACGTCTG172550.11874065403356944Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTAGTATG158420.10901706410894275TruSeq Adapter, Index 7 (97% over 37bp)
CACTACATGGATAACCGTAGTAATTCTAGAGCTAATACATGCAAGGAGTC149600.10294756211777448No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTAGTGTG146150.10057343718925628TruSeq Adapter, Index 7 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)