FastQCFastQC Report
Fri 20 Dec 2024
1E3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23136181
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA12256935.297732586030513Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC5618372.428391271662337No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT4482791.937566964919578Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG2619311.1321272080297091No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2369861.0243090681214846Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC1761900.761534498714373No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1682280.7271208675277913No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT1444940.6245369536139088No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG1382110.5973803541734048Illumina DpnII expression Sequencing Primer (100% over 23bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1255110.5424879758677545Illumina RNA PCR Primer (100% over 45bp)
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG1243710.5375606285237827Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1231790.5324085249851737Illumina RNA PCR Primer (100% over 50bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT933330.403407113732383Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC766250.33119121950161096Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC733270.3169364900801908Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAA731520.31618009904054606No Hit
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT703430.3040389422956191No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT702380.3035851076718323Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG630630.27257307504639594No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG612060.2645466855571367No Hit
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG509750.22032590426224624Illumina RNA PCR Primer (100% over 33bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT478470.20680595470790966No Hit
GCTATTTTCAGTAAGGACCAACTAAGCCAGATCGTCGGACTGTAGAACTC475630.20557844010642895Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA458000.19795834066132176No Hit
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG456140.19715440504204218No Hit
ACGGAGACTCCTTGCATGTATTAGCTCTAGAATTACTACGGTTATCCATG455590.1969166821438681No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTAGATCGTCGGACTG441150.1906753755081705No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT433210.1872435213054393Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCAGTTCAATTGTAAAAGACTACAGTCAGATCGTCGGACTGTAGAACT432310.18685452019933627No Hit
TCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC430510.1860765179871302Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT429830.18578260604029678No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGGATCGTCGGACT426320.18426550172649495No Hit
TTTCATTTGTATCCTCACGGTTGACCCCTGATCGTCGGACTGTAGAACTC394710.17060291843325395Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC346300.149678981159423No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA344940.14909115726575617Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACAGACCCAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG328460.1419681147895584No Hit
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT300910.1300603587082933No Hit
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT283640.1225958597056273No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC282320.12202532475000952No Hit
GCTTCCTATGAGGTCACATGAGAACCAATCAGATCGTCGGACTGTAGAAC264650.11438793636685328No Hit
GCTTTCAGAGTTAGAAAGAACAGAATATGAGATCGTCGGACTGTAGAACT247120.10681105926686864No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT243820.10538472187782418Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGTGATCAGAGTAAGCCACCTCCATCAGCAGATCGTCGGACTGTAGAACT241930.10456781955500781No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGATCGTCGGAC240980.10415720727634349No Hit
GCTCCAGATTGAGACAACAGATACGTAAGATCGTCGGACTGTAGAACTCT235400.10174540041850468Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAACT234570.10138665495398744No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC232510.10049627464446272No Hit
GATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGA231600.10010295130384742Illumina DpnII expression Sequencing Primer (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)