FastQCFastQC Report
Fri 20 Dec 2024
1E3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23136181
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC11532414.984578051148545Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT5471752.36501866924364No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG4286041.8525270008909422Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC2500271.0806753283958144No Hit
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC2220280.9596570842871605Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT1674510.7237624913117684No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1627230.7033269665378223No Hit
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA1388000.5999261503011235No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA1320590.5707899674540063Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA1175490.5080743446811727Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTGGGGGG987090.42664344647027097TruSeq Adapter, Index 21 (97% over 43bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC866020.3743141532303884Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTAACGAGTAGATAAATGAAGAGTAGATCGGAAGAGCACACGTCTGAACT723460.3126963780236678Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAAGATCGGAAGAGCACACG707710.30588885866686466No Hit
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT694490.3001748646416623Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC677640.2928918994885111No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG668420.2889067992682111Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA624340.26985438953818697No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA599440.2590920256026697No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTGGGGGGGGGGG495080.21398518623276674TruSeq Adapter, Index 21 (97% over 39bp)
GAGGTCCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT475910.20569946267277212TruSeq Adapter, Index 21 (100% over 37bp)
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA454080.19626402473251744No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC453230.19589663479897568No Hit
TGGCTTAGTTGGTCCTTACTGAAAATAGCAGATCGGAAGAGCACACGTCT450190.1945826755072499Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG439820.19010051831804048No Hit
TAGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGC421790.18230752949244303No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGCAGATCGGAAGAGCACACGTC412560.17831810703763082No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC411900.1780328395598219No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAG409870.17715542595383393No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG408420.17652870194955686Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAAAATCTTTCTTCTGAGGCGGAAGATCGGAAGAGCACACGTCTGAACT402180.17383162761390913Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTACATGGATAACCGTAGTAATTCTAGAGCTAATACATGCAAGGAGTC389250.16824297838956223No Hit
AGGGGTCAACCGTGAGGATACAAATGAAAAGATCGGAAGAGCACACGTCT375920.16248143978472507Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATGATTGAAATCTTCATGAACGACACAAAAGGCCAAAAAAAAAAAAAAA337700.14596185947888288No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCAGATCGGAAGAGCACACGT332080.14353276368299506No Hit
CACCCCTCCCCGCACATTGACCTCTTAGATCGGAAGAGCACACGTCTGAA329640.1424781384620046Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATACGATCCACTGAGATTCATCCTGTGTTTTTGGGTCTGTAGATCGGAA320720.1386227052770723No Hit
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC287200.12413457519199042No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT267760.11573215130016487No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC265410.11471642618978474No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGCAGATCGGAAGAGCACACGT249990.10805154057188608No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGCAGATCGGAAGAGCACACGTC234510.1013607215469139No Hit
TGCTGATGGAGGTGGCTTACTCTGATCACCAGATCGGAAGAGCACACGTC234480.10134775484337713No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGA232730.10059136380373235No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGATCGGAAGAGCACACGTCTG232300.10040550771970534Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)