FastQCFastQC Report
Fri 20 Dec 2024
1E1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18473203
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC5777283.1273840275560225No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA5394362.920099995653163Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG3600451.9490123071781325Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1996411.0807059284738008No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT1743970.9440539358550869No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1576330.8533062728753643Illumina RNA PCR Primer (100% over 50bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1254330.6789997381612707Illumina DpnII expression Sequencing Primer (100% over 22bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1186960.6425306970318033Illumina RNA PCR Primer (100% over 45bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1163120.6296255175672567No Hit
TTCCGCCCCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA969150.5246247767644842Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG950500.514529072191758No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC830790.44972709930162086No Hit
TCTAGAGCGTCTACCAATTTCGCCACATCCGCGATCGTCGGACTGTAGAA612160.33137729282788697No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT562820.3046683349931249Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCCGGAGATCCTAACCACCTAGACTACGTTGGAGATCGTCGGACTGTAG551590.2985892592638104No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTAGATCGTCGGACTG498550.26987740025376217No Hit
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT496910.2689896278409326Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC494360.26760925000391106No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC464700.25155356112310356Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT405700.2196154072469187No Hit
GGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG381420.20647204493990565Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAA361220.1955372871721271No Hit
ACGGAGACTCCTTGCATGTATTAGCTCTAGAATTACTACGGTTATCCATG354310.1917967338961197No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC280210.15168457792619935No Hit
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT273510.1480577028250055No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA270520.14643914214551748No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG266490.14425760383838146Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCCGGAGATCCTAACCACCTAGACTACGATGGAGATCGTCGGACTGTAG262350.14201651982062882No Hit
AATCTAGAGCGTCTACCAATTTCGCCACATCCGCGATCGTCGGACTGTAG248020.13425933770120968No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACGATCGTC246320.133339085809862No Hit
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG236290.12790959965091056No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT232310.125755127575873No Hit
TGTCCGGCTAGGGCTCGAACCTAGAGTCTTCTGGAGATCGTCGGACTGTA225870.12226899688159112No Hit
AACCAGCGCTCTAACCAACTGAGCTACAATCACCGATCGTCGGACTGTAG224000.12125671980110866No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT222940.12068291568062127No Hit
AGGAAGTACTCTACCCCTGAGCTACATCGGCGATCGTCGGACTGTAGAAC222430.12040684011321695No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG216630.1172671571897954No Hit
ACAGACCCAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG202630.1096886122022261No Hit
AAAGCAGATGCTCTAGCCAGCTGAGCTAATTCCCCGATCGTCGGACTGTA201240.10893617094988887No Hit
TTTTTTTCAAGAATCCATCCCGCCTATCAATCACTGATCGTCGGACTGTA198550.10748000766299162No Hit
ACCAGCGCTCTAACCAACTGAGCTACAATCACCGATCGTCGGACTGTAGA196020.10611045631880947No Hit
AGTTCAGCGGGTAGCCTCGTCGGCCGTCAGGTCAAAGATCGTCGGACTGT193050.10450272213216084No Hit
TTTTTTTTCAAGAATCCATCCCGCCTATCAATCACTGATCGTCGGACTGT187690.10160122205120574No Hit
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC187190.10133055973022113No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)