FastQCFastQC Report
Fri 20 Dec 2024
1E1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18473203
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT5839553.1610923130114466No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC5304982.8717163991539527Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA3547381.9202842084288252Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT2007541.0867308717389181No Hit
AGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGCA1725530.9340719094571742No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1244170.6734998797988633Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1169200.6329167713904297No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGGGGG1147840.6213540770379669TruSeq Adapter, Index 5 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGGGGGGGGGG1132960.6132991663654646TruSeq Adapter, Index 5 (97% over 41bp)
TGAAAATCTTTCTTCTGGGGCGGAAAGATCGGAAGAGCACACGTCTGAAC951630.5151407690371832Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC939830.5087531382619462No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAGATCGGAAGAGCACACGT826110.4471936999772048No Hit
GCGGATGTGGCGAAATTGGTAGACGCTCTAGAAGATCGGAAGAGCACACG608170.32921740750642975No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG558000.30205915021883323Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAACGTAGTCTAGGTGGTTAGGATCTCCGGCTAGATCGGAAGAGCACA547020.296115405650011No Hit
TAGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTAGATCGGAAGAGC494010.26741978637922187No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT493500.26714371081181754No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG488480.264426261109132Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT460410.24923127840905554Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC405110.21929602570815687No Hit
GGCTGTTTGGTCTAGTGGTATGATTCCAGATCGGAAGAGCACACGTCTGA383470.20758176045594257Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATTAATGCTGTAGGTTAAAGGCCAAAAAAAAAGATCGGAAGAGCACACG363200.19660910996322617No Hit
CACTACATGGATAACCGTAGTAATTCTAGAGCTAATACATGCAAGGAGTC285750.15468351644270895No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG276600.14973039596869042No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC273610.1481118352892024No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG270730.14655282032033104No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA263990.14290429223345838Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCATCGTAGTCTAGGTGGTTAGGATCTCCGGCTAGATCGGAAGAGCACA259540.14049539757669527No Hit
GCGGATGTGGCGAAATTGGTAGACGCTCTAGATTAGATCGGAAGAGCACA247320.13388041045183122No Hit
GTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGG244130.13215358484394937No Hit
AGTGGTGATGACACTTAGTTACCTGTCTTAGCTTTGATGCTGATATCTCA231290.1252029764410644No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC231240.12517591020896593No Hit
TATGATTGAAATCTTCATGAACGACACAAAAGGCCAAAAAAAAAAAAAAA230730.12489983464156161No Hit
TCCAGAAGACTCTAGGTTCGAGCCCTAGCCGGACAAGATCGGAAGAGCAC224390.12146783641147667No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC222100.1202282029813671No Hit
GCCGATGTAGCTCAGGGGTAGAGTACTTCCTAGATCGGAAGAGCACACGT221330.1198113830070508No Hit
TCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGA216300.11708852005794555No Hit
GGTGATTGTAGCTCAGTTGGTTAGAGCGCTGGTTAGATCGGAAGAGCACA215790.11681244449054123No Hit
GATACGATCCACTGAGATTCATCCTGTGTTTTTGGGTCTGTAGATCGGAA200790.10869257486100271No Hit
GGGGAATTAGCTCAGCTGGCTAGAGCATCTGCTTTAGATCGGAAGAGCAC199870.10819455619039103No Hit
AGTGATTGATAGGCGGGATGGATTCTTGAAAAAAAAGATCGGAAGAGCAC196980.10663012797509992No Hit
GGTGATTGTAGCTCAGTTGGTTAGAGCGCTGGTAGATCGGAAGAGCACAC192940.10444317642154421No Hit
TTTGACCTGACGGCCGACGAGGCTACCCGCTGAACTAGATCGGAAGAGCA192100.10398846372229005No Hit
AGTGATTGATAGGCGGGATGGATTCTTGAAAAAAAAAGATCGGAAGAGCA187050.10125477428034543No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)