FastQCFastQC Report
Wed 18 Dec 2024
1E12_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E12_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11655061
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA5578994.786753153844497Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG3887643.3355809978171718Illumina Small RNA Adapter 1 (100% over 26bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG3204002.7490203611975947Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG3120742.6775835836466233Illumina RNA PCR Primer (100% over 37bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA2507232.1511942322738595Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG1449301.24349413529453Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC1349411.1577888781534478No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1198991.0287290645668865No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1104510.9476655677735192No Hit
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG1056290.9062929829367689Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACAGGGTCGAGTTCGACTATCTTGATCGGATCGTCGGACTGTAGAACTC924800.7934750405853732Illumina DpnII expression Sequencing Primer (100% over 21bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA864320.7415834202841153Illumina RNA PCR Primer (100% over 45bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA800470.6868003522246688Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT784200.6728407513268271No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG667560.5727640550315438Illumina Small RNA Adapter 1 (100% over 26bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT662190.5681566145385254Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG648080.5560502857942999No Hit
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT637040.5465780058980386Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT624410.5357415117775874Illumina RNA PCR Primer (100% over 50bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT585070.5019879346834821Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG554810.4760249646055048Illumina RNA PCR Primer (100% over 36bp)
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG549600.4715548035312728Illumina DpnII expression Sequencing Primer (96% over 29bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT537730.46137038665005703Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT509480.43713198927058383Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG484340.41556196059377126Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA474950.4075053747037446No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC471260.4043393681079833Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG463090.3973295377862029Illumina Small RNA Adapter 1 (100% over 26bp)
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT420340.36065019307921253Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATGATCGTCGGACTG394160.33818784818028835No Hit
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC387890.33280821095659646Illumina RNA PCR Primer (100% over 30bp)
GACAGGGTCGAGTTCGACTATCTTGATCGGGATCGTCGGACTGTAGAACT383970.3294448651963297No Hit
GCCTCGACCACGGCCAGTCCGATCGTCGGACTGTAGAACTCTGAACGTGT373980.3208734814858541Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAACACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTCGGACTGTAG373830.32074478203074186No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT356030.3054724466907552No Hit
CGCGACCTCGGAAAGGGAGAGAGAACCCCCCCTCGCCTCGACCACGGCCA347220.2979134986938292No Hit
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC333800.2863991874431202Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG316430.271495790541122Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT306370.26286434708492734Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT265430.22773797580295804Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC253240.21727900008416942Illumina RNA PCR Primer (100% over 30bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC246740.21170202369597207Illumina RNA PCR Primer (100% over 32bp)
GCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT244320.2096256724868278Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG241440.20715464294867267Illumina RNA PCR Primer (100% over 37bp)
GAGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAAC239110.20515551141259578Illumina DpnII expression Sequencing Primer (100% over 26bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG219530.1883559425386105Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGATTAAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGG210170.18032509653960627No Hit
GCCTCGACCACGGCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA208310.1787292232962144Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC204770.1756919161555654No Hit
AACAGGGTCGAGTTCGACTATCTTGATCGGGATCGTCGGACTGTAGAACT203850.1749025594975436No Hit
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG202920.1741046228758477Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT198810.1705782578057721Illumina RNA PCR Primer (100% over 44bp)
ACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACGTGT191550.16434920417833934Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG189080.16222995315082434No Hit
AACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC181770.15595799970502083Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA177240.15207127616063099Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACTGCAAGCAGTCCACCACGGTCGATCGTCGGACTGTAGAACTCTGA174240.14949728705838605Illumina DpnII expression Sequencing Primer (100% over 24bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCCGGGAT173090.14851059123585883No Hit
TGACAGGGTCGAGTTCGACTATCTTGATCGGATCGTCGGACTGTAGAACT171900.14748957555863498No Hit
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA170070.1459194422062656Illumina DpnII expression Sequencing Primer (100% over 25bp)
AACAGGGTCGAGTTCGACTATCTTGATCGGATCGTCGGACTGTAGAACTC169190.1451644054029404Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTTGTCAGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT168100.1442291893624581Illumina RNA PCR Primer (100% over 35bp)
TGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG163950.14066850443768592Illumina Small RNA Adapter 1 (100% over 26bp)
ACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGACT160590.13778563664317156No Hit
TGACAGGGTCGAGTTCGACTATCTTGATCGGGATCGTCGGACTGTAGAAC158990.1364128424553076No Hit
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG158260.13578650510709467Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGGATCGTCGGACTGTAGAACT154740.13276635789379396No Hit
GCCAGTCCGGGCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC154150.1322601400370191Illumina RNA PCR Primer (100% over 30bp)
TCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTG152100.13050124748381842Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT150310.1289654339861456Illumina RNA PCR Primer (100% over 31bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCT147800.126811863103934Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT147270.12635712502920407Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT145190.12457249258498089No Hit
GGCCAGTCCGGGCCGGATCGATCGTCGGACTGTAGAACTCTGAACGTGTA143720.12331123792488087Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTCT142700.1224360816301176Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCGATCGTCGGA141910.12175826449985976No Hit
GACCGCGGTCCGTTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA141090.12105470747857947Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT140560.12059996940384954No Hit
AACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA139610.11978487285480531Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG139580.11975913296378285No Hit
GGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT138840.11912421565189577Illumina RNA PCR Primer (100% over 35bp)
AATCATAGATCAGACAGTTCACTAGCAAAGTTGCCACGATCGTCGGACTG135760.11648158684025764No Hit
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC134940.11577802981897736Illumina RNA PCR Primer (100% over 32bp)
CCTCGCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAA132950.11407061704782154Illumina DpnII expression Sequencing Primer (100% over 25bp)
GACAGGGTCGAGTTCGACTATCTTGATCGGGCTGTGACCAACCGCCTATT128670.11039839259528543No Hit
AGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA127050.10900843848007317No Hit
CAGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACG127010.10897411862537656Illumina Small RNA Adapter 1 (100% over 26bp)
ACAGGGTCGAGTTCGACTATCTTGATCGGGATCGTCGGACTGTAGAACTC126330.10839068109553439Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA126060.10815902207633234No Hit
ACTCGTTCTCAAAGTAGTTCAGCAGAAAGATCGTCGGACTGTAGAACTCT125280.10748978490974866Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG123650.10609125083086224Illumina Small RNA Adapter 1 (100% over 26bp)
GCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC123430.10590249163003093Illumina RNA PCR Primer (100% over 30bp)
GGCCAGTCCGGGCCGGATCCAAGATCGTCGGACTGTAGAACTCTGAACGT122990.10552497322836835Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTGTCGGCGCCAGTCAGGGATCGTCGGACTGTAGAACTCTGAACGTGTA122540.10513887486303161Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACACTCAGTTAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT120340.10325128285471866Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA120120.10306252365388736Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC119710.10271074514324721Illumina RNA PCR Primer (100% over 30bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC119380.10242760634200027Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTA118820.10194712837624789No Hit
TGCGCTCTAACCGACTGAGCTACAGACCCGATCGTCGGACTGTAGAACTC118070.10130363110068664Illumina DpnII expression Sequencing Primer (100% over 21bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGGCTGTGAGCAACCGCCTATT117880.10114061179087781No Hit
GCCAGTCCGGGCCGGATCCAACCGATCGTCGGACTGTAGAACTCTGAACG117450.10077167335288935Illumina Small RNA Adapter 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)