FastQCFastQC Report
Wed 18 Dec 2024
1E12_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E12_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11655061
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC5267774.519727524377607Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC3702713.176911729591119Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA3021552.592478923962732Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCAT2939132.5217628633603892TruSeq Adapter, Index 3 (97% over 36bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT2333612.0022289029632705Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA1380221.1842237462335032Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA1309431.123486183384197No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1181171.0134395692995515No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG1090200.9353876397558109No Hit
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA978840.8398411642804786Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGATCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT904320.7759032749807144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG766210.6574053966770316No Hit
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA733220.6291000965160114Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA650130.5578091783475008No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG636260.545908768731455Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC636190.5458487089857359Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG611130.5243473200183165Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG552440.4739915132147314Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCA526010.45131466922395347TruSeq Adapter, Index 3 (97% over 36bp)
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA516780.4433953627527132Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG509470.4371234093069097Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC479060.4110317397738201No Hit
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC475480.4079601127784745Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGTGGACTGCTTGCAGTGCCAGATCGGAAGAGCACACGTCTGAACTC463580.39774995600623625Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT461430.39590526381629404Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC457150.3922330393637579Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGACTGGCCGTGGTCGAGGCGAGGGGGGGTTCTCTCTCCCTTTCCGAGG424020.36380761971129966No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG409450.35130661263806345Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGAGC383740.3292475260318243No Hit
CCGATCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTC378860.32506050375883916No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGTGTTCTAGATCGGAAGAGCACA367860.3156225437172744No Hit
CTTTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT359780.30868993306856135Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAG351420.30151708343697214Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG349400.29978393077479387No Hit
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT319420.2740611996796928Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA300920.25818826688251567Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC281320.24137153808118209Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTAGGGGG274320.23536556350927723TruSeq Adapter, Index 3 (97% over 36bp)
ATGGGGGACAAGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC254000.21793107732340483Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT237710.20395431649821483Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC231200.1983687601463433Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCA230180.19749360385158TruSeq Adapter, Index 3 (97% over 36bp)
CGTGGTGGACTGCTTGCAGTGCTCAGATCGGAAGAGCACACGTCTGAACT229920.1972705247960521Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTAGTGGG225120.19315214223246022TruSeq Adapter, Index 3 (97% over 36bp)
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCAT224630.19273172401242689TruSeq Adapter, Index 3 (97% over 36bp)
CCTGTGAATAGGCGGTTGGTCACAGCCCGATCAAGATAGTCGAACTCGAC224330.19247432510220236No Hit
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC207960.17842892456761916Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTAGATCGGAA207460.17799992638391168No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT200730.17222561083120885No Hit
CCGATCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTC198420.17024363922248026No Hit
CTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA197980.16986612082081767Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC187960.1612689972193196Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGTGGTGGACTGCTTGCAGTAGATCGGAAGAGCACACGTCTGAACTCCAG180000.15443934613469631Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTCT175300.15040676320784593Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGA170190.1460224017703554No Hit
CCTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC169920.14579074275115333No Hit
TCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA169390.1453360046764234Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGATCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGTC167440.14366291175996418No Hit
GACCGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAA166210.14260757622804376Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC162970.13982766799761923Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGATCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTCT162690.13958742901474305Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGTGGACTGCTTGCAGTGCAAGATCGGAAGAGCACACGTCTGAACTC162370.13931287017717023Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGACAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTC158700.13616402350875728TruSeq Adapter, Index 3 (97% over 36bp)
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC158110.13565780565198243No Hit
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTAGATCGGAAGAG157140.1348255491755899No Hit
CCGATCAAGATAGTCGAACTCGACCCTGTCAAGATCGGAAGAGCACACGT155810.133684414006928No Hit
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA154590.1326376584386817Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTC152610.13093882563120005No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTGGGGGG149800.12852785583876394TruSeq Adapter, Index 3 (97% over 36bp)
CCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT146730.12589380699079997Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC143130.12280502006810604TruSeq Adapter, Index 5 (97% over 35bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC142040.12186980402762371No Hit
CTTTCCGAGGTCGCGGTCGGAAGATCGGAAGAGCACACGTCTGAACTCCA141990.12182690420925296Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTG139690.1198535125641985Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCC139240.11946741419886177Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA138630.11894403641473862No Hit
CGACCAAGATAGTCGAACTCGACCCTGTAGATCGGAAGAGCACACGTCTG138210.11858367794042435Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCTAGATCGGAAGAGCACA138000.1184034987032672No Hit
GGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAG137900.11831769906652569No Hit
CCGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA134800.11565791032753926Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT134190.11513453254341612Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGGCAACTTTGCTAGTGAACTGTCTGATCTATGATTAGATCGGAAGAGC133200.11428511613967528No Hit
CGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTC131440.11277504253302492TruSeq Adapter, Index 3 (97% over 36bp)
CAGCTGTGACCCTCGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT129890.11144514816353171Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTGGATCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCA127760.10961761590093781TruSeq Adapter, Index 3 (97% over 36bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCTAGATCGGAAGAGCAC125210.1074297251640296No Hit
CGGACTGGCCGTGGTCGAGGCGAGGAGATCGGAAGAGCACACGTCTGAAC124140.10651166905089557Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGATCAAGATAGTCGAACTCGACCCTGTAGATCGGAAGAGCACACGTCT123920.10632290985006428Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG122110.10476993642504318No Hit
TTTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTG120080.10302820379919075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGTGAATAGGCGGTTGCTCACAGCCCGACCAAGATAGTCGAACTCGAC120000.10295956408979756No Hit
GTTGGATCCGGCCCGGACTGGCTAGATCGGAAGAGCACACGTCTGAACTC119330.10238470652362952Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGACCGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTC118830.10195570833992204Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCAC118270.10147523037416964No Hit
TTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTCC117340.10067729375247371Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)