FastQCFastQC Report
Wed 18 Dec 2024
1E11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9776022
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG2846132.91133755631892Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG2419082.474503432991456Illumina Small RNA Adapter 1 (100% over 26bp)
GCCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG1958302.0031665231522595Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC1677581.7160149598681345Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTG1608891.6457512063700348Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1382391.4140618750653384Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG1078751.103465192692897Illumina Small RNA Adapter 1 (100% over 26bp)
AGTTAAGAGCACACTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1015641.0389092823236281Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCT935760.9571991552392168Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC764290.7818006137874894Illumina RNA PCR Primer (100% over 30bp)
ACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG738770.7556959262162054Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT706080.72225696709766Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCCGAGGGTCACAGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTA677850.6933801908383594Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGT609980.6239552243233495Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG562500.5753874121805372No Hit
GGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA490860.502106071365224No Hit
GACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC437800.44783041609358076Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCTCGACCACGGCCAGTCCGATCGTCGGACTGTAGAACTCTGAACGTGT403550.4127957158852547Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG387250.3961222673189565Illumina Small RNA Adapter 1 (100% over 26bp)
TGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG364650.3730044797362363Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG351720.35977824108824635Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA339410.3471862072323487Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT333800.34144767677486815Illumina RNA PCR Primer (100% over 31bp)
ACCTCGACCACGGCCAGTCCGGATCGTCGGACTGTAGAACTCTGAACGTG329580.3371309925448204Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC299420.3062799981423937Illumina RNA PCR Primer (100% over 30bp)
GACCGCGGTCCGTTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA293970.30070513343771116Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAACG291780.2984649584462883Illumina Small RNA Adapter 1 (100% over 26bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGAC289580.2962145543453155No Hit
GGCCAATCCACGGCCGATTCCTTCCCGGGATCGTCGGACTGTAGAACTCT283010.28949402937104685Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCTCGACCACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG269810.27599160476521023Illumina RNA PCR Primer (100% over 33bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA265050.27112254861946916Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCTCTTGTCCCCCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT260640.2666115113079737Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCT259810.265762495215334Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTAAGAGCACACTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA259420.2653635599428888Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG246270.2519122808848016Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGT238810.24428136516059396Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT232650.2379802336778702Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC232420.23774496415822302No Hit
ATCCAACCGCTTCAAGTCTGATCGTCGGACTGTAGAACTCTGAACGTGTA230600.2358832662201456Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAACCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTA222930.22803753919539055Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGT221560.22663615118705746Illumina DpnII expression Sequencing Primer (96% over 28bp)
GACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCCTTCCGATCGT221460.22653386009155874No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGT207420.2121721902835325No Hit
GACCGCGGTCCGTTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT206030.21075034405609971Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACG203910.20858177283152596Illumina Small RNA Adapter 1 (100% over 26bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG196280.2007769622449704Illumina RNA PCR Primer (100% over 37bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC195850.2003371105343257No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT195460.19993817526188057Illumina RNA PCR Primer (100% over 50bp)
CAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAA184320.18854294722331846Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCCTCTATTCTTGTTCGCCTAACCGGATCGTCGGACTGTAGAACTCTGA182680.18686537325713876Illumina DpnII expression Sequencing Primer (100% over 24bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT177230.1812905085524562Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACAGGGTCGAGTTCGACTATCTTGGTCGGATCGTCGGACTGTAGAACTC177130.18118821745695743Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGA175990.18002209896827154Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCTCGACCACGGCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA173820.1778023821959484Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA170710.1746211291259369Illumina RNA PCR Primer (100% over 45bp)
GCCTCGACCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG168290.17214568461486687Illumina RNA PCR Primer (100% over 34bp)
ACCCGAGGGTCACAGCTGGGCCAATCCACGGCCGATTCGATCGTCGGACT167950.17179789489017105No Hit
AGCACACTCGGTCTCGGCGAGGAAGAACAGCTCGGGATCGTCGGACTGTA159760.16342025416882247No Hit
GGCCTCTATTCTTGTTCGCCTAACCGTGATCGTCGGACTGTAGAACTCTG153570.15708843535744907Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACAGGGTCGAGTTCGACTATCTTGGTCGGGATCGTCGGACTGTAGAACT151760.1552369665289215No Hit
CAGTTAAGAGCACACTCGGTCTCGATCGTCGGACTGTAGAACTCTGAACG147920.15130898846176902Illumina Small RNA Adapter 1 (100% over 26bp)
GGCCTCTATTCTTGTTCGCCTAACCGTAAACGATCGTCGGACTGTAGAAC146600.14995874600118533No Hit
ATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC145850.14919156278494464Illumina RNA PCR Primer (100% over 30bp)
GCCTCGACCACGGCCAGTCGATCGTCGGACTGTAGAACTCTGAACGTGTA145260.14858804532150194Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAACACCGCCCCAACGACCGGCAGCAGTCGCGGGATCGTCGGACTGTAG143680.1469718460126215No Hit
TCAGTTAAGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA141030.1442611319819043Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG140750.14397471691450775Illumina Small RNA Adapter 1 (100% over 26bp)
AATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT129970.1329477368197412Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC129690.13266132175234466Illumina RNA PCR Primer (100% over 32bp)
GCCAATCCACGGCCGATTCGATCGTCGGACTGTAGAACTCTGAACGTGTA128580.1315258905923084Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCAATCCACGGCCGATTCCTTCCGATCGTCGGACTGTAGAACTCTGAA126700.12960281799693169Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACCGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGA126360.1292550282722359Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG126150.1290402169716885Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCAGTCCGGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTC124720.1275774543060562No Hit
ACCTACCTTCCACGGCACCTCCGACGCCCAGTTCGATCGTCGGACTGTAG121720.12450872144109332No Hit
TCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTGAACGTG119960.1227083981603151Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCAGTCCGGGCCGGATCCAACCGCTTCAAGTCTGATCGTCGGACTGTA119900.12264702350301585No Hit
GCCTCTATTCTTGTTCGCCTAACCGGATCGTCGGACTGTAGAACTCTGAA118970.12169571631487737Illumina DpnII expression Sequencing Primer (100% over 25bp)
CGCCCCAACGACCGGCAGCAGTCGGATCGTCGGACTGTAGAACTCTGAAC115130.11776773824772489Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCACTGCAAGCAGTCCACCACGGTCGATCGTCGGACTGTAGAACTCTGA114240.11685734749778591Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGTCTCGGCGAGGAAGAACAGCTCGGCGATCGTCGGACTGTAGAACTCTG113360.11595718585739681Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT112210.11478083825916105Illumina RNA PCR Primer (100% over 44bp)
AGGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTA111270.11381930196147268No Hit
GGCACTGCAAGCAGTCCACCACGGATCGTCGGACTGTAGAACTCTGAACG109130.11163027251779917Illumina Small RNA Adapter 1 (100% over 26bp)
GACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC108870.11136431566950238Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCAGTCCGGGCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT108850.11134385745040262Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCGGATCCAACCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAG107630.11009590608531772No Hit
GGCCAGTCCGGGCCGGATCCAAGATCGTCGGACTGTAGAACTCTGAACGT104560.10695556945350573Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCAACCGCTTCAAGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA103380.10574853452662034Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCAACCGCTTCAAGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAG103130.10549280678787343Illumina DpnII expression Sequencing Primer (96% over 30bp)
CTCGCCTCGACCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT102970.1053291410350754Illumina RNA PCR Primer (100% over 31bp)
TCCCGGTCCGACCGCGACCTCGGAAAGGATCGTCGGACTGTAGAACTCTG102110.10444943761378607Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAACGT100320.10261842700435822Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCGGGCTGTAACAGTCCGGAGCCGAGACTCCAGGATCGTCGGACTGTA99940.10222972084146292No Hit
GGGCCGGATCCAACCGCTTCAAGTCTGATCGTCGGACTGTAGAACTCTGA98030.10027596091743657Illumina DpnII expression Sequencing Primer (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)