FastQCFastQC Report
Wed 18 Dec 2024
1E11_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E11_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9776022
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA2573772.6327375286184913Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC2262072.3138961839488497Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCA1806121.8474999340222433Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGGGCTGAGACTTGAAGCGGTTAGATCGGAAGAGCACACGTCTGAACT1583111.619380561950454Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGGCTGAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGA1504701.5391741139698745Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1237001.265340851319688Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAACTC1016461.039748069306718Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC933330.954713481618597Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTG888380.9087336341919034Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC705180.7213363472381712Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGCTGTGACCCTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC684680.7003666726609249Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC634040.6485664619003517Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGCTGTGACCCTCGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT616910.6310439972414137Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG563590.5765023851214738Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCAGATCGGAAGAGCACA562600.575489703276036No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCAGATCGGAAGAGCAC492160.503435855606708No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTCT423200.4328959161507615Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGGATCCGGCCCGGACTGGCTAGATCGGAAGAGCACACGTCTGAACTC372320.38085020676099135Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAG371050.37955110984815704Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGGCTGAGACTTGAAGCAAGATCGGAAGAGCACACGTCTGAACTCCA348800.35679134109968247Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC317590.3248662901945188Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC315910.3231477997901396Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACTGGCCGTGGTCGAGGTAGATCGGAAGAGCACACGTCTGAACTCCA308100.3151588652316863Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG307330.3143712237963458TruSeq Adapter, Index 2 (100% over 35bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCGGAAGA281650.28810287047226363No Hit
CCGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA275080.281382345497995Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGACTGCTGCCGGTCGTTGGGGCAGATCGGAAGAGCACACGTCTGAACTC274010.2802878307761582Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCCGAGGTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC272740.27898873386332396Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTG270190.2763803109281055Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC247380.2530477120448379Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGA247200.25286358807294007TruSeq Adapter, Index 7 (97% over 37bp)
CTTTCCGAGGTCGCGGTCGGACCGGGAAAGATCGGAAGAGCACACGTCTG243730.24931408705913305Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGGGGACAAGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC241230.24675680967166397Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAGTGTGCTCTTAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA239530.24501786104818504Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG230000.235269519647153No Hit
CTTTCCGAGGTCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC229740.2350035627988562Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAG219620.2246517039343815Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGGAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTCAGATCG217150.22212511387556208No Hit
AGACTTGAAGCGGTTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGT213050.21793117896011283Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC211890.2167446022523272Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGGATCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCC205040.20973766221066195Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCA203670.20833627420232892No Hit
GAGACTTGAAGCGGTTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT202130.206760991331648Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGGCTGAGACTTGAAGCGGTAGATCGGAAGAGCACACGTCTGAACTC192670.1970842536974651Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT191420.19580561500373056No Hit
CGAACGGACCGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC191160.19553965815543375Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATAT180190.18431832497921957TruSeq Adapter, Index 7 (97% over 37bp)
CGGTTAGGCGAACAAGAATAGAGGCCAGATCGGAAGAGCACACGTCTGAA174850.17885598047958565Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACCAAGATAGTCGAACTCGACCCTGTTAGATCGGAAGAGCACACGTCT170950.17486662775513395Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGGCTGAGACTTGAAGCGGTTGAGATCGGAAGAGCACACGTCTGAAC170270.17417104830574234Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGAA168420.17227866303901526Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG167040.17086704592113233Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATCGGCCGTGGATTGGCCCAGCTGTGACCCTCGGGTAGATCGGAAGAG164430.16819724832861466No Hit
CTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA161000.16468866375300711Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGAGCTGTTCTTCCTCGCCGAGACCGAGTGTGCTAGATCGGAAGAGCAC158750.16238711410428494No Hit
GTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAA154250.15778401480684065TruSeq Adapter, Index 7 (97% over 37bp)
CCGACCAAGATAGTCGAACTCGACCCTGTCAGATCGGAAGAGCACACGTC148380.15177952750106333No Hit
ACGGTTAGGCGAACAAGAATAGAGGCCAGATCGGAAGAGCACACGTCTGA148250.15164654907691494Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTTACGGTTAGGCGAACAAGAATAGAGGCCAGATCGGAAGAGCACACGT143210.14649107786377732No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGTGTTCTAGATCGGAAGAGCACA142650.14591824772898424No Hit
GAGACCGAGTGTGCTCTTAACTGAGATCGGAAGAGCACACGTCTGAACTC135280.1383793939907255Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGGATCCGGCCCGGACTGGCAAGATCGGAAGAGCACACGTCTGAACTC133680.1367427364627453Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACTGGCCGTGGTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT133310.13636425940939986Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAATCGGCCGTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC132440.13547432687856062Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGCTCTTAACTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA127640.13056435429462004Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCCGGACTGGCAGATCGG125720.12860036526104382No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA119950.12269816905076524Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCTGCCGGTCGTTGGGGCGGTAGATCGGAAGAGCACACGTCTGAA119420.1221560262446218Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAACTGGGCGTCGGAGGTGCCGTGGAAGGTAGGTAGATCGGAAGAGCACA119170.12190029850587489No Hit
AGACTTGAAGCGGTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCAC118850.12157296700027885No Hit
GGAAGGAATCGGCCGTGGATTGGCCAGATCGGAAGAGCACACGTCTGAAC118610.12132746837108181Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGGATCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGG118470.12118426083738355TruSeq Adapter, Index 7 (97% over 36bp)
GAATCGGCCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC118050.12075463823628875Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAATCGGCCGTGGATTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT117440.1201306625537463Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTTAGGCGAACAAGAATAGAGGCAGATCGGAAGAGCACACGTCTGAAC112050.11461717250636301Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTCCGAGGTCGCGGTCGGAAGATCGGAAGAGCACACGTCTGAACTCCA109880.11239745573403986Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCCTAGATCGGAAGAGCAC109670.11218264443349248No Hit
CGTGGTGGACTGCTTGCAGTGCCAGATCGGAAGAGCACACGTCTGAACTC108460.11094492217795746Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACCGTGGTGGACTGCTTGCAGTGCTAGATCGGAAGAGCACACGTCTGAA108090.11056644512461204Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGGCTGAGACTTGAAGCGGTTGGATCCGGCTAGATCGGAAGAGCACA107250.10970719992242242No Hit
CTCGGGCTGAGACTTGAAGCGGTCAGATCGGAAGAGCACACGTCTGAACT107220.10967651259377281Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGAGCTGTTCTTCCTCGCCGAGACCAGATCGGAAGAGCACACGTCTGA107020.10947193040277527Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCTGCCGGTCGTTGGGGCGAGATCGGAAGAGCACACGTCTGAACT105780.10820352081859064Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGGCCCGGACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC100910.1032219444678009Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGAGTCTCGGCTCCGGACTGTTACAGCCCGGCTAGATCGGAAGAGCAC98440.10069535440898147No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)