FastQCFastQC Report
Wed 18 Dec 2024
1E10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12332113
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA151194512.260226613233272Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG12118559.82682367571559Illumina RNA PCR Primer (100% over 37bp)
GAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG6497465.2687321304953985Illumina RNA PCR Primer (100% over 36bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC4042663.278156792757251No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC2373621.9247472026894337No Hit
GGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG1968861.5965309432373835Illumina Small RNA Adapter 1 (100% over 26bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1185530.9613356608068706Illumina RNA PCR Primer (100% over 45bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT1162500.9426608400360911Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT978670.793594739198384No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT940960.7630160378841809No Hit
ACTCGTTCTCAAAGTAGTTCAGCAGAAGATCGTCGGACTGTAGAACTCTG913370.7406435539473243Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT905560.7343104948843722Illumina RNA PCR Primer (100% over 50bp)
AAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG806330.6538457764699367Illumina RNA PCR Primer (100% over 37bp)
CCACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGT695120.5636665833340969Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA601720.48792935971313267No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC583220.4729278753770745No Hit
CCTTCCTTAGGGAGTTACACAAGATCGTCGGACTGTAGAACTCTGAACGT527410.4276720461448902Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTAGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC495660.4019262554600335Illumina RNA PCR Primer (100% over 32bp)
AAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG448820.3639441189032244Illumina RNA PCR Primer (100% over 36bp)
TAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG430300.3489264167462624Illumina RNA PCR Primer (100% over 37bp)
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG426390.34575583275956034Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATAGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG409430.33200312063309834No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC364080.2952292117336259No Hit
GAGGTCCGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT359890.29183157825427Illumina RNA PCR Primer (100% over 35bp)
ACTCATCCTAAGTAGACTTTTCACATTGAAGATCGTCGGACTGTAGAACT350830.2844849053848274No Hit
AGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG325200.2637017678965478Illumina Small RNA Adapter 1 (100% over 26bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG325120.26363689661293244Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC321020.26031224332764386Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAAGTTCGGATCTACGGGTGATCGTCGGACTGTAGAACTCTGAACGTGT306760.2487489370232011Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA283520.22990382913293125Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTTCTGAATCACTGTCCATCCTGATCGTCGGACTGTAGAACTCTGAACGT259220.21019917673475744Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG257580.20886931542064202Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAGACCGAAAAACACAGGATGAATCTCAGTGGATCGTATCGATCGTCGG254520.20638798882235349No Hit
CTTGAGAGATCGGAGCTAGTCCCCGTTGTAGATCGTCGGACTGTAGAACT242900.19696543487721851No Hit
TAGGTCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG235370.19085942530692024Illumina RNA PCR Primer (100% over 36bp)
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT233560.1893917125151221Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCTTGAGAGATCGGAGCTAGTCCCCGTTGTAGATCGTCGGACTGTAGAAC229730.18628599981203545No Hit
AATGAGGTCAAAGTAGCGGATGAGGGGTGGATCGTCGGACTGTAGAACTC227170.18421011873634308Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT226770.18388576231826614Illumina RNA PCR Primer (100% over 31bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC222760.18063408922704488No Hit
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG221060.1792555744502179Illumina Small RNA Adapter 1 (100% over 26bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC175600.14239246753577428No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT138480.11229219193823477No Hit
ACCGTCAAAAGTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC135060.10951894456367696Illumina RNA PCR Primer (100% over 32bp)
AATGGAGGTCCCACCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGTA134410.10899186538430194Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAAC132550.10748360804024419No Hit
TGCCAGTCCGGGCCGGATCCAACGATCGTCGGACTGTAGAACTCTGAACG129110.10469414284478257Illumina Small RNA Adapter 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)