FastQCFastQC Report
Wed 18 Dec 2024
1E10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12332113
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC143199211.611894895870643Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAAT11353549.206483917233TruSeq Adapter, Index 23 (97% over 38bp)
CCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAA6133644.9737137504335225TruSeq Adapter, Index 23 (97% over 38bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3983943.230541270583557No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG2350141.9057074809483174No Hit
GTTGGATCCGGCCCGGACTGGCCAGATCGGAAGAGCACACGTCTGAACTC1863911.5114279280444478Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1115410.904475980717984Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG964240.7818935814162585No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC915120.7420626132764109No Hit
TTCTGCTGAACTACTTTGAGAACGAGTAGATCGGAAGAGCACACGTCTGA874400.709043129916179Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTGGGGGG837960.6794942602293703TruSeq Adapter, Index 23 (97% over 38bp)
CGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAAT755130.612328154956089TruSeq Adapter, Index 23 (97% over 38bp)
ACCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC659070.5344339611549132Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG588550.4772499246479496No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT575820.4669272816426512No Hit
TTGTGTAACTCCCTAAGGAAGGAGATCGGAAGAGCACACGTCTGAACTCC488000.39571483005386016Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGACTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAA463040.3754749895658595TruSeq Adapter, Index 2 (97% over 36bp)
CCGGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAA423890.3437286051465795TruSeq Adapter, Index 23 (97% over 38bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA395280.32052901234362674Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAAT392090.31794226990946317TruSeq Adapter, Index 23 (97% over 38bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT353770.2868689250576929No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACGCATCGATGAAGAACGCAGCC343300.2783788958145291No Hit
TCCGGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTA338440.27443796533489434TruSeq Adapter, Index 23 (97% over 38bp)
TTCAATGTGAAAAGTCTACTTAGGATGAGTAGATCGGAAGAGCACACGTC337660.2738054703196443No Hit
GTTGGATCCGGCCCGGACTGGCTAGATCGGAAGAGCACACGTCTGAACTC313020.25382511496610516Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA310750.25198439229351854Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT302380.2451972342452587Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG291030.23599362088232567Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAAAATCTTTGCTCTGAAGTGGATAGATCGGAAGAGCACACGTCTGAAC275330.223262631472806Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATACGATCCACTGAGATTCATCCTGTGTTTTTCGGTCTGTAGATCGGAA252630.20485540474693997No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA246440.19983598917719939Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGATGGACAGTGATTCAGAAAAGATCGGAAGAGCACACGTCTGAACTCC243220.1972249200116801Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTGGGGGGGGGGG238480.1933812964574684TruSeq Adapter, Index 23 (97% over 34bp)
TACAACGGGGACTAGCTCCGATCTCTCAAGAGATCGGAAGAGCACACGTC231820.18798076209648745No Hit
CACCCCTCATCCGCTACTTTGACCTCATTAGATCGGAAGAGCACACGTCT223350.18111251494370834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC220880.17910961406208328Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACAACGGGGACTAGCTCCGATCTCTCAAGGAGATCGGAAGAGCACACGT220310.17864740616632366No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT216980.17594713898583317No Hit
CCGGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAA216750.17576063404543893TruSeq Adapter, Index 23 (97% over 38bp)
GTTGGATCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA212990.17271168371551573TruSeq Adapter, Index 7 (100% over 35bp)
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC207660.16838963444464058Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG170230.13803798262309144No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTAGGGGG155980.12648278522910064TruSeq Adapter, Index 23 (97% over 38bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC134820.1093243307128308No Hit
CGGACCTCAGATCGGAAGAGCACACGTCTGAACTCAGTCACCAACTAATC132820.10770254862244612Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TCTCGGTGGGACCTCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT129660.10514013291963835Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT129060.10465359829252294No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTTGAACT127480.10337239044111905No Hit
AACTTTTGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAA127030.10300748947078249TruSeq Adapter, Index 2 (97% over 36bp)
GTTGGATCCGGCCCGGACTGGCAAGATCGGAAGAGCACACGTCTGAACTC124350.10083430146966704Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)