FastQCFastQC Report
Fri 20 Dec 2024
1D9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12411917
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2123401.710775217075654No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG1579581.27263177799207Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1377311.109667426876928No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1154830.9304203371646782Illumina RNA PCR Primer (100% over 50bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT891530.7182854993310058Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA844290.6802253028279194Illumina RNA PCR Primer (100% over 45bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT825090.6647562983220079No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA717870.578371576284308No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT534150.4303525394183671No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG529860.4268961837240774No Hit
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA521840.4204346516335873Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCATCGATCGTCGGAC519590.4186218776680508No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC489820.39463686391070774No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG459340.37007981925757316No Hit
AGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA358730.2890206242919607No Hit
TGCGGGGACTAGCGCGAACGATCGTCGGACTGTAGAACTCTGAACGTGTA298220.24026908977879888Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTTTGTAAAGTTTACATTGGTAGTTCAGAGATCGTCGGACTGTAGAACT281540.2268303921142882No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC270600.2180162822551907Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT268250.21612294055785258Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG264640.21321444543981402Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA236480.19052657216447708Illumina DpnII expression Sequencing Primer (100% over 25bp)
CTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACT219800.17708787449996644No Hit
GAGCTAGCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC217500.1752348166685291Illumina RNA PCR Primer (100% over 32bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT216170.17416326583556754Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT213950.1723746621895715Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT194120.15639808097330976Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA193920.15623694550970652No Hit
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT188700.15203130990966182No Hit
AGTGAATCCATGTACGATTTTCGATGTGAGATCGTCGGACTGTAGAACTC185920.1497915269655767Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC173420.1397205604903739No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG165900.13366186705889188No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT161160.12984295657149494No Hit
AGTTCGGCGGGTGATCCAGGCTGATCCGAGGTCCGGATCGTCGGACTGTA160930.12965765078835123No Hit
AGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC158520.12771596845193212Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCT158470.12767568458603132Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG154870.1247752462411729No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC154100.1241548747063004No Hit
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT149290.12027956680664235No Hit
GGTAAAGCTTACATAAGATTTCTTCATCCATGATCGTCGGACTGTAGAAC139080.1120536013896967No Hit
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG136070.10962851266246784Illumina DpnII expression Sequencing Primer (100% over 23bp)
GATCCTGTCTGTCCTGTTCGAGATCGATTAGATCGTCGGACTGTAGAACT134890.1086778134272087No Hit
AGTCGAATGCTCTACCACTGAGCTACACCCCCGATCGTCGGACTGTAGAA134840.1086375295613079No Hit
GCAGGGTCCTAACCACTAGACGATCGCGGCCGATCGTCGGACTGTAGAAC130970.1055195583405851No Hit
ATCAACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATC128600.10361010309688663No Hit
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG125200.10087080021563147Illumina RNA PCR Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)