FastQCFastQC Report
Fri 20 Dec 2024
1D9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12411917
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2002551.6134091131933932No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA1459231.1756685127688171Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1332021.073178301143973No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC801980.6461370955026529Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC776160.6253345071514739No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGCAC686980.5534842039307869No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGG561380.45229113278794886TruSeq Adapter, Index 20 (97% over 43bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC515690.4154797361277875No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA501980.40443390009778507No Hit
GATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGA500900.4035637685943275No Hit
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC478690.3856696753611871Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT473890.3818024242347093No Hit
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC432520.348471553588378No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGGGGGG428140.3449426869354669TruSeq Adapter, Index 20 (97% over 39bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCAC343190.27650039876998855No Hit
GTTCGCGCTAGTCCCCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT270620.21803239580155104Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTGAACTACCAATGTAAACTTTACAAAGTAGATCGGAAGAGCACACGTC265240.21369785183062376No Hit
GGCTGTTTGGTCTAGTGGTATGATTCCAGATCGGAAGAGCACACGTCTGA257610.20755053389415992Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTAACAATGACCTGATCTGGAGACTCCAGATCGGAAGAGCACACGTCT252250.20323210346959297Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG247640.19951793103353815Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAAAATCTTTCTTCTGAGACGGAAAGATCGGAAGAGCACACGTCTGAAC214980.17320450982712823Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTCC204450.1647207276684174Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCAAGACCTGAGTTACCACTGACAAGCAGAGATCGGAAGAGCACACGTC198970.16030561596568846No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGATCGGAAGAGCACACGTCTG198050.15956439283311355Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG196740.15850895554651226TruSeq Adapter, Index 19 (100% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC183990.14823656974180538No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC182020.14664938542531342No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG178670.1439503664099591Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACATCGAAAATCGTACATGGATTCACTAGATCGGAAGAGCACACGTCT176440.1421537059907829Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTAGATCGG169460.13653007831102962No Hit
CGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGATCGGAA161310.1299638081691974No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGGG154710.1246463378702903TruSeq Adapter, Index 20 (97% over 43bp)
CGGACCTCGGATCAGCCTGGATCACCCGCCGAACTAGATCGGAAGAGCAC153440.1236231276764097No Hit
TAGTAACAATGACCTGATCTGGAGACTCTAGATCGGAAGAGCACACGTCT152480.12284967745111411Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACTTGGGGAGGGTTATCCACTAGATCGGAAGAGCACACGTC151930.1224065549262052No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGTAGATCGGAAGAGCACACGT145120.1169198923905147No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCAGATCGGAAGAGCACACGTCTG144450.11638008858744382Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCATATGGTCTAGTGGCTAGGATCCGGCAGATCGGAAGAGCACACGTC140770.1134151960571441No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACCAGATCGGAAGAGCACACGT129840.10460914297122675No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTCTTAAATTCAGTTGATAGAT127010.10232907616124085No Hit
GGGGGTGTAGCTCAGTGGTAGAGCATTCGACTAGATCGGAAGAGCACACG126990.10231296261488051No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA126100.10159590980184609Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAATCGATCTCGAACAGGACAGACAGGATCAGATCGGAAGAGCACACGTC125730.10129780919418008No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)