FastQCFastQC Report
Fri 20 Dec 2024
1D8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10088635
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2953872.9279183953032297Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2896962.8715083854257784Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA944450.9361524130866069Illumina DpnII expression Sequencing Primer (100% over 24bp)
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA920580.912492126040837Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA816800.8096238985749806Illumina RNA PCR Primer (100% over 45bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG640440.6348133320315384No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGGATCGTCGGA611320.6059491695358193No Hit
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC496370.4920090775412135No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC485630.48136343519217417No Hit
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT473590.4694292141602903Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT473020.46886422196858146Illumina RNA PCR Primer (100% over 50bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGATCGTCGGAC448870.44492639489881436No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG432330.4285317091955453No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGGATCGTCGGACT411730.40811269314431536No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGATCGTCGGACTGTAGAACTCTGAA382370.37901063919945566Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG356840.3537049362971304No Hit
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC345580.3425438624749532No Hit
TTTTTTTGGGCTTTTACCTACAGCATAAAGAAGATCGTCGGACTGTAGAA342940.3399270565344073No Hit
GCTTTCAGAGTTAGAAAGAACAGAATATGAGATCGTCGGACTGTAGAACT326200.32333412795685446No Hit
TAAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTG292670.2900987100831778Illumina DpnII expression Sequencing Primer (100% over 23bp)
CATCAAGTTTTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG288430.28589596114836147Illumina RNA PCR Primer (100% over 33bp)
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC275360.27294078931391613Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT272730.2703338955170843Illumina DpnII expression Sequencing Primer (100% over 22bp)
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG232750.23070514494775554No Hit
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC223060.22110027768870616Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCTGAATATGTACTTCGAAGTTCCATGAGAGATCGTCGGACTGTAGAACT220880.2189394303590129No Hit
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT206000.20419016051229927No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT203700.20191036745803567No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT199470.19771753066693365No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT198850.1971029777566539Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATGATCGTCGGACTGTAG193900.1921964666181302No Hit
TAAGAGGTCAATGTGCGGGGAGGGGTGATCGTCGGACTGTAGAACTCTGA191820.19013474072557884Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT189470.18780538695274435No Hit
GCTTCCTATGAGGTCACATGAGAACCAATCAGATCGTCGGACTGTAGAAC188290.18663575399447002No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT179780.17820051969369494No Hit
TATCAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTC177850.1762874759568564Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACGATCGTC177000.17544494374115033No Hit
CAAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGA176210.17466188438772937Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACTTGGTTGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT158960.15756343648075283No Hit
ACTGCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAAC153340.15199281171337847No Hit
TAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA149100.14779006277856221Illumina DpnII expression Sequencing Primer (100% over 24bp)
AAGAGGTCAATGTGCGGGGAGGGGTGGGATCGTCGGACTGTAGAACTCTG147990.14668981482628718Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACTTTTCGAATTTTTCAAAGGTAGATGTAAGATCGTCGGACTGTAGAACT143790.1425267144663277No Hit
AGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGAA142160.14091103504091484Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGAACGT141090.1398504356634966Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATTCAGAGACTCTAGTCT139920.13869071484893647No Hit
TATGAGGTCAATGTTCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTG137080.1358756660341067Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTTCATTTGTATCCTCACGGTTGACCCCTGATCGTCGGACTGTAGAACTC135000.13381394014155532Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATGAGGTCAATGTTCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA131600.13044381127873098Illumina DpnII expression Sequencing Primer (100% over 24bp)
TTTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAA129240.1281045453621823No Hit
TCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC122750.12167156409167346Illumina DpnII expression Sequencing Primer (100% over 26bp)
GATTTTCCTCAGGTTGAGCTTCGTCTTCAGATCGTCGGACTGTAGAACTC121650.12058122828311263Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG120780.11971887177997817Illumina RNA PCR Primer (100% over 33bp)
AAAACTACCATTATTAGAATCCTTTGATCGTCGGACTGTAGAACTCTGAA119200.11815275307313626Illumina DpnII expression Sequencing Primer (100% over 25bp)
CAGGCTGATCCGAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTA118930.11788512519285314Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCTGATCCGAGGTCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG118100.11706241726457543Illumina DpnII expression Sequencing Primer (96% over 30bp)
CTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACTC111220.11024286238921321Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC110880.10990584950293077Illumina DpnII expression Sequencing Primer (100% over 26bp)
TATGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTG110400.10943006660464968Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTTTTTTTGGGCTTTTACCTACAGCATAAAGAAGATCGTCGGACTGTAGA110020.10905340514351049No Hit
ACTCGTCGTATGTCCCTTAAATTCCATAAGATCGTCGGACTGTAGAACTC109410.10844876437694495Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA108060.1071106249755294Illumina DpnII expression Sequencing Primer (100% over 24bp)
TTTTTTTTTCCATCGCGAGTAAACAATTAAGATCGTCGGACTGTAGAACT107520.10657536921496316No Hit
ACTTGGTCGACAGGTTCTGCACATAAAAGAGATCGTCGGACTGTAGAACT106830.10589143129868411No Hit
AAAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTG105420.10449381903498342Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC101350.1004595765433084No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)